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関連する概念動画

Separation of Sister Chromatids02:17

Separation of Sister Chromatids

At the transition from prophase to metaphase, there is a reduction in cohesion along the chromosomal arms, resulting in the resolution of sister chromatids. However, residual cohesin connections remain to hold the sister chromatids together until the transition from metaphase to anaphase. The residual connection prevents any premature separation of sister chromatids, blocking the risks of aneuploidy within the daughter cells.
At the onset of anaphase, separase, a proteolytic enzyme, is...
The Spindle Assembly Checkpoint02:19

The Spindle Assembly Checkpoint

The spindle assembly checkpoint is a molecular surveillance mechanism ensuring the fidelity of chromosome segregation during anaphase. The checkpoint monitors the completion of all the prerequisite steps before chromosome segregation to determine whether the segregation process should proceed or be delayed.
Many proteins function together to control the spindle assembly checkpoint. Mutations affecting these proteins may allow cells to proceed into anaphase prematurely, resulting in the...
Anaphase Promoting Complex00:50

Anaphase Promoting Complex

The stepwise destruction of specific proteins is necessary for the progression and completion of the cell cycle. Such proteins are ubiquitinated by ubiquitin ligases and then subsequently destroyed by the proteasome. The SCF (Skp1/Cullin/F-box) and the anaphase-promoting complex (APC) are two important ubiquitin ligases involved in cell cycle progression. While SCF is active throughout the cell cycle, APC gets activated during metaphase to anaphase transition. Cdc20 or Cdh1 binds to APC and...
Separation of Sister Chromatids02:17

Separation of Sister Chromatids

At the transition from prophase to metaphase, there is a reduction in cohesion along the chromosomal arms, resulting in the resolution of sister chromatids. However, residual cohesin connections remain to hold the sister chromatids together until the transition from metaphase to anaphase. The residual connection prevents any premature separation of sister chromatids, blocking the risks of aneuploidy within the daughter cells.
At the onset of anaphase, separase, a proteolytic enzyme, is...
The Spindle Assembly Checkpoint02:19

The Spindle Assembly Checkpoint

The spindle assembly checkpoint is a molecular surveillance mechanism ensuring the fidelity of chromosome segregation during anaphase. The checkpoint monitors the completion of all the prerequisite steps before chromosome segregation to determine whether the segregation process should proceed or be delayed.
Many proteins function together to control the spindle assembly checkpoint. Mutations affecting these proteins may allow cells to proceed into anaphase prematurely, resulting in the...
Anaphase Promoting Complex00:50

Anaphase Promoting Complex

The stepwise destruction of specific proteins is necessary for the progression and completion of the cell cycle. Such proteins are ubiquitinated by ubiquitin ligases and then subsequently destroyed by the proteasome. The SCF (Skp1/Cullin/F-box) and the anaphase-promoting complex (APC) are two important ubiquitin ligases involved in cell cycle progression. While SCF is active throughout the cell cycle, APC gets activated during metaphase to anaphase transition. Cdc20 or Cdh1 binds to APC and...

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関連する実験動画

Updated: Jul 16, 2026

Immunofluorescence Analysis of Endogenous and Exogenous Centromere-kinetochore Proteins
05:35

Immunofluorescence Analysis of Endogenous and Exogenous Centromere-kinetochore Proteins

Published on: March 3, 2016

セパラーゼは,Cdc14を通してINCENP-Aurora Bアナフェーズスパインドルの機能を調節する.

Gislene Pereira1, Elmar Schiebel

  • 1The Paterson Institute for Cancer Research, Wilmslow Road, Manchester M20 4BX, UK.

Science (New York, N.Y.)
|November 8, 2003
PubMed
まとめ

フォスファターゼCdc14はSli15を脱リン酸化し,INCENP-Aurora複合体を誘導して,ミトーシス中にマイクロチューブルをスパインドルさせます. Cdc14によるこの調節は,染色体分離と細胞分裂に極めて重要です.

科学分野:

  • 細胞生物学 細胞生物学
  • 分子生物学は分子生物学である.
  • 遺伝学 遺伝学とは

背景:

  • インナー・セントロメア・ライク・プロテイン (INCENP) は,オーロラBキナーゼと複合体を形成する.
  • INCENP-Aurora複合体は,ミトーシス中の染色体分離,スパインドル機能,およびサイトキネシスに不可欠です.
  • INCENP-Aurora複合体のシフトを誘発するメカニズムは,アナフェーズにおけるキネトコアからスピンドルマイクロチューブルにシフトするメカニズムは不明である.

研究 の 目的:

  • イーストINCENP-Aurora複合体 (Sli15-Ipl1) の調節におけるフォスファタゼCdc14の役割を調査する.
  • ミトーシス中のSli15-Ipl1複合体の局所化と機能にCdc14がどのように影響を与えるかを決定する.

主な方法:

  • イーストにおけるCdc14,Sli15,およびIpl1の相互作用を調査した.
  • Cdc14によるSli15の脱リン酸化を分析した.
  • Cdc14の活性化時にSli15-Ipl1複合体の移転を研究した.

主要な成果:

  • Cdc14は,酵母INCENP-Aurora複合体の成分であるSli15をデフォスフォリレートする.
  • Cdc14による脱酸化は,Sli15-Ipl1複合体をマイクロチューブルをスピンドルするように指示します.

さらに関連する動画

Identification of Cyclin-dependent Kinase 1 Specific Phosphorylation Sites by an In Vitro Kinase Assay
12:26

Identification of Cyclin-dependent Kinase 1 Specific Phosphorylation Sites by an In Vitro Kinase Assay

Published on: May 3, 2018

Mass Spectrometry Analysis to Identify Ubiquitylation of EYFP-tagged CENP-A (EYFP-CENP-A)
09:02

Mass Spectrometry Analysis to Identify Ubiquitylation of EYFP-tagged CENP-A (EYFP-CENP-A)

Published on: June 10, 2020

関連する実験動画

Last Updated: Jul 16, 2026

Immunofluorescence Analysis of Endogenous and Exogenous Centromere-kinetochore Proteins
05:35

Immunofluorescence Analysis of Endogenous and Exogenous Centromere-kinetochore Proteins

Published on: March 3, 2016

Identification of Cyclin-dependent Kinase 1 Specific Phosphorylation Sites by an In Vitro Kinase Assay
12:26

Identification of Cyclin-dependent Kinase 1 Specific Phosphorylation Sites by an In Vitro Kinase Assay

Published on: May 3, 2018

Mass Spectrometry Analysis to Identify Ubiquitylation of EYFP-tagged CENP-A (EYFP-CENP-A)
09:02

Mass Spectrometry Analysis to Identify Ubiquitylation of EYFP-tagged CENP-A (EYFP-CENP-A)

Published on: June 10, 2020

  • セパラーゼ媒介によるCdc14の活性化は,Sli15の脱リン酸化と再局所化を誘発するのに十分である.
  • Cdc14は,Sli15-Ipl1複合体を通して,スパインドルミッドゾーンアセンブリを調節する.

    • 結論:

    • Cdc14は,ミトーシス中のINCENP-オーロラ複合体の局所化を調節する上で重要な役割を果たします.
    • Cdc14媒介による脱酸化は,微小管をスパインドルする複合体を指向するための重要なイベントです.
    • Cdc14の機能は,ミトスの出口を超えて,スパインドルミッドゾーンアセンブリの調節を含みます.