転写因子 Eyes absentは,タンパク質チロシンフォスファタゼである
Tina L Tootle1, Serena J Silver, Erin L Davies
1Whitehead Institute for Biomedical Research, Cambridge, Massachusetts 02142, USA.
Nature
|November 25, 2003
まとめ
Eyes absent (Eya) タンパク質はチロシンフォスファタゼとして作用し,遺伝子転写を調節する. この内在的なフォスファタゼ活動は,ドロソフィラの目の発達に不可欠であり,転写制御のための新しいメカニズムを明らかにしています.
科学分野:
- バイオケミストリー バイオケミストリー
- 分子生物学は分子生物学である.
- 発達生物学 発達生物学とは
背景:
- 翻訳後の改変は,タンパク質の機能を動的に調節する.
- 転写因子のリン酸化は,それらの安定性,局所化,および活性に影響を与えます.
- The Eyes absent (Eya) タンパク質は,進化的に保存された転写因子です.
研究 の 目的:
- Eyes absent (Eya) タンパク質の酵素活性を調べるために.
- タンパク質の機能と発達におけるEyaの潜在的フォスファターゼ活性が果たす役割を決定する.
- Eyaが転写調節を調節するメカニズムを解明する.
主な方法:
- タンパク質チロシンフォスファタゼの活性を検査するための生化学的測定法.
- 培養ドロソフィラ細胞を用いたインビトロおよび細胞ベースの実験.
- サイト・ディレクテッド・ミュータゲネシス (SITE-DIRECTED MUTAGENESIS) は,推定のフォスファターゼ活性部位を破壊するために行われる.
- 変異株を用いたドロソフィラの眼の発達におけるEyaの役割の分析.
主要な成果:
- Eyes absent (Eya) は,固有のタンパク質チロシンフォスファタゼ活性を示しています.
- Eyaは自己触媒的に自己脱化することができる.
- フォスファタゼ活性部位を非活性化する変異は,目の特異化と発達におけるEyaの機能を損なう.
- Eyaは,ホロ酸脱ホロゲナーゼ (HAD) スーパーファミリーに属しています.
結論:
- Eyes absent (Eya) は,非チオール基のタンパク質チロシンフォスファタゼとして機能する.
- Eyaの固有のフォスファタゼ活動は,目の発達における転写共同活性化剤としての役割に不可欠です.
- この発見は,フォスファタゼを含む転写因子を介して転写調節を微調整するための新しいメカニズムを明らかにしています.
関連する概念動画
Master Transcription Regulators
7.4K
Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
7.4K
Transcription Factors
80.4K
Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
80.4K
General Transcription Factors
6.2K
Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
6.2K
Eukaryotic Transcription Inhibitors
10.4K
Certain biochemical processes, such as embryonic development and cell growth regulation, depend on the repression of specific genes. DNA binding proteins known as eukaryotic transcription inhibitors regulate the repression of gene expression in eukaryotes. The presence of these inhibitors at the required location and time in the cell is triggered by the presence of hormones and additional signals from other cells.
Eukaryotic transcription inhibitors usually contain two distinct domains, a...
Eukaryotic transcription inhibitors usually contain two distinct domains, a...
10.4K
Epistasis Analysis
5.4K
Although Mendel chose seven unrelated traits in peas to study gene segregation, most traits involve multiple gene interactions that create a spectrum of phenotypes. When the interaction of various genes or alleles at different locations influences a phenotype, this is called epistasis. Epistasis often involves one gene masking or interfering with the expression of another (antagonistic epistasis). Epistasis often occurs when different genes are part of the same biochemical pathway. The...
5.4K
RNA Polymerase II Accessory Proteins
10.2K
Proteins that regulate transcription can do so either via direct contact with RNA Polymerase or through indirect interactions facilitated by adaptors, mediators, histone-modifying proteins, and nucleosome remodelers. Direct interactions to activate transcription is seen in bacteria as well as in some eukaryotic genes. In these cases, upstream activation sequences are adjacent to the promoters, and the activator proteins interact directly with the transcriptional machinery. For example, in...
10.2K


