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RNAi Screening to Identify Postembryonic Phenotypes in C. elegans
Published on: February 13, 2012
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天然RNAiによるCaenorhabditis elegans細菌系におけるトランポゾン静止は,自然RNAiによるものである
Titia Sijen1, Ronald H A Plasterk
1Hubrecht Laboratory, Uppsalalaan 8, 3584 CT, Utrecht, The Netherlands.
Nature
|November 25, 2003
まとめ
RNA干渉 (RNAi) は,ネマトード菌株の転移可能な要素を沈黙させます. このプロセスは,Tc1要素の読み込みトランスクリプションによって誘発され,トランポザースの遺伝子発現を抑制する二重鎖RNAを生成します.
科学分野:
- 遺伝学 遺伝学とは
- 分子生物学は分子生物学である.
- エピジェネティクス エピジェネティクス
背景:
- Tc1のようなトランスポーザブル要素 (TEs) は,ゲノム内で移動することができる.
- *Caenorhabditis elegans*では,Tc1トランポゾンは生殖系では静止されているが,体細胞では活性化している.
- ゲルムラインサイレンシングの喪失は,しばしばRNA干渉 (RNAi) の障害と相関する.
研究 の 目的:
- ネマトード菌株のRNAi媒介トランポゾンサイレンシングのメカニズムを解明する.
- Tc1サイレンシングにおける読み込み転写と二重鎖RNA (dsRNA) の役割を調査する.
主な方法:
- Tc1-derived dsRNAsの検出,特にターミナル反転繰り返しから.
- 転置可能な要素の読み込みトランスクリプションの分析.
- Tc1配列に融合したゲルムラインレポーター遺伝子におけるトランスゲンサイレンシングの評価.
- *変異*遺伝子 (mut-7,mut-16,pk732) を用いた遺伝分析.
主要な成果:
- Tc1要素の読み込みトランスクリプションから生じるトランポゾン由来dsRNAの証拠.
- Tc1.1.に特異的な小さな干渉RNAの検出
- 機能的な変異遺伝子に依存するTc1融合レポーター遺伝子の生殖線静止.
- Tc1末端の反転繰り返しの"スナップバック"によるdsRNAの形成.
結論:
- RNAiの監視は,分散したTc1コピーの偶然の読み込みトランスクリプションによって開始されます.
- dsRNA形成は,ターミナル・リピート・スナップバックを通じて,RNAiを誘発する.
- このRNAiメカニズムは,トランスポゼーゼの遺伝子発現を効果的に静止させ,生殖系におけるTc1のトランスポジションを防止します.
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