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関連する概念動画

MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...
Regulation of Angiogenesis and Blood Supply01:24

Regulation of Angiogenesis and Blood Supply

Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits.  Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl hydroxylase and factor...
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
Autoregulation of Blood Flow01:17

Autoregulation of Blood Flow

Autoregulation mechanisms are characterized by their inherent capacity for self-regulation without necessitating specific nervous stimulation or endocrine control. These mechanisms facilitate the adjustment of blood flow and, therefore, perfusion specific to each tissue region. This self-regulation encompasses chemical signals and myogenic controls.
Chemical Signaling in Autoregulation
Chemical signaling operates at the precapillary sphincter level, inciting either contraction or relaxation.

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関連する実験動画

Updated: Jul 20, 2026

Purification and microRNA Profiling of Exosomes Derived from Blood and Culture Media
10:45

Purification and microRNA Profiling of Exosomes Derived from Blood and Culture Media

Published on: June 14, 2013

マイクロRNAは,血球形成系統の微分化を調節する.

Chang-Zheng Chen1, Ling Li, Harvey F Lodish

  • 1Whitehead Institute for Biomedical Research, Nine Cambridge Center, Cambridge, MA 02142, USA.

Science (New York, N.Y.)
|December 6, 2003
PubMed
まとめ

マイクロRNA (miRNA) は遺伝子発現を調節する. この研究では,特定のmiRNA,miR-181が,マウスの血液形成におけるBリンパ球細胞の発達を促進することを発見しました.

科学分野:

  • 分子生物学は分子生物学である.
  • 発達生物学 発達生物学について
  • 遺伝学 遺伝学とは

背景:

  • マイクロRNA (miRNA) は,遺伝子発現に関与する小さな,調節性RNA分子です.
  • これらの分子は,植物や動物を含む様々な生物の発達において重要な役割を果たします.
  • 特定のmiRNAは,メッセンジャーRNAをターゲットにすることで,転写後の遺伝子発現を調節することが知られている.

研究 の 目的:

  • 哺乳類の血液形成におけるマイクロRNAの役割を調査する.
  • 血液形成細胞で発現する特定のmiRNAを特定し,発達の過程でそれらの動的調節を行う.
  • 血液型幹細胞と原始細胞の分化に対するマイクロRNAの機能的影響を決定する.

主な方法:

  • 血液形成細胞におけるmiRNAの識別と特徴付け.
  • 血液形成と系統のコミットメント中のmiRNA発現パターンの分析.
  • 特定のmiRNAs (例えば,miR-181) が血液形成性幹細胞/祖先細胞でエクトピック発現する.
  • Bリンパ球細胞の微分化の評価 in vitroおよびin vivo.

主要な成果:

  • 3つのmiRNAは,早期の血液形成の間に動的に調節される血液形成細胞の特定の発現と特定されました.

さらに関連する動画

Fast and Simplified Method for High Through-put Isolation of miRNA from Highly Purified High Density Lipoprotein
09:48

Fast and Simplified Method for High Through-put Isolation of miRNA from Highly Purified High Density Lipoprotein

Published on: July 27, 2016

Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis
11:44

Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis

Published on: March 30, 2019

関連する実験動画

Last Updated: Jul 20, 2026

Purification and microRNA Profiling of Exosomes Derived from Blood and Culture Media
10:45

Purification and microRNA Profiling of Exosomes Derived from Blood and Culture Media

Published on: June 14, 2013

Fast and Simplified Method for High Through-put Isolation of miRNA from Highly Purified High Density Lipoprotein
09:48

Fast and Simplified Method for High Through-put Isolation of miRNA from Highly Purified High Density Lipoprotein

Published on: July 27, 2016

Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis
11:44

Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis

Published on: March 30, 2019

  • miR-181は,マウスの骨髄のBリンパ球細胞で好ましく発現することが判明しました.
  • miR-181の子宮外発現は,微分化アッセイおよび成人マウスにおけるB系細胞の分数を増加させた.

    • 結論:

    • マイクロRNAは,マウスの血液形成を制御する分子回路の不可欠な構成要素である.
    • miR-181は,B型リンパ球細胞の発達を促進する上で重要な役割を果たします.
    • これらの発見は,他のマイクロRNAが脊椎動物の発達において同様の調節機能を持つ可能性があることを示唆しています.