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Updated: Jul 19, 2026

Calibration-free In Vitro Quantification of Protein Homo-oligomerization Using Commercial Instrumentation and Free, Open Source Brightness Analysis Software
08:22

Calibration-free In Vitro Quantification of Protein Homo-oligomerization Using Commercial Instrumentation and Free, Open Source Brightness Analysis Software

Published on: July 17, 2018

In vivoで合成されたタンパク質は,単指数的な光衰退の動態学を持つ

Jaap Broos1, Francesco Maddalena, Ben H Hesp

  • 1Department of Biochemistry, Groningen Biomolecular Science and Biotechnology Institute (GBB), and Ultrafast Laser & Spectroscopy Laboratory, University of Groningen, Nijenborgh 4, 9747 AG Groningen, The Netherlands. j.broos@chem.rug.nl

Journal of the American Chemical Society
|January 8, 2004
PubMed
まとめ

トリプトファンのアナログである5-フッ素トリプトファンは,タンパク質の単指的光分解を示し,データ分析を簡素化します. この簡素化により,複雑なタンパク質システムにおける共振エネルギー伝送 (RET) を使用して距離を正確に測定できます.

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Protein Denaturation

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Bacterial Protein Maturation

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