アラビドプシス・タリアナのヴァルナライゼーションは,PHD指タンパク質VIN3によって媒介されます
Sibum Sung1, Richard M Amasino
1Department of Biochemistry, University of Wisconsin-Madison, 433 Babcock Drive, Madison, Wisconsin 53706, USA.
Nature
|January 9, 2004
まとめ
長期にわたる寒さを要求するプロセスであるヴェルナライゼーションは,FLC (FLOWERING LOCUS C) 遺伝子を安定的に抑制することによって,アラビドプシス・タリアナのような植物の開花を可能にします. 新しく特定された遺伝子は,寒さの持続期間を測定し,ヒストンの修正を通じて,この重要な言語化状態を確立します.
科学分野:
- 植物生物学 植物生物学
- 分子遺伝学 分子遺伝学
- エピジェネティクス エピジェネティクス
背景:
- 2年生と冬の1年生では,最初の成長期に開花が抑制されます.
- 長期にわたる冬の寒さに晒されるヴェルナライゼーションは,この開花ブロックを克服するために不可欠です.
- アラビドプシス・タリアナでは,FLOWERING LOCUS C (FLC) 遺伝子が開花抑制剤として作用し,高発現で最初の季節の開花を防ぐ.
研究 の 目的:
- ベルナライゼーションのための冷たい期間を測定するのに関与する遺伝子を特定するために.
- 言語化状態を確立する分子メカニズムを理解する.
- ヴァルナライゼーションが安定したFLC遺伝子抑制にどのようにつながるのかを解明する.
主な方法:
- アラビドプシス・タリアナの遺伝子スクリーンは,変異した言語化反応を持つ突然変異者を特定するために行われます.
- FLCを中心とした遺伝子発現の分子分析.
- クロマチン免疫プレシピテーション (ChIP) は,FLCの位置におけるヒストンの改変を研究する.
主要な成果:
- 寒さの持続時間を感知し, vernalisation を開始するために重要な新しい遺伝子の識別.
- ヴァルナライゼーションがFLCのミトーシス的に安定した抑制を誘導することを実証した.
- ヴァルナライゼーション中のFLC遺伝子サイレンシングは,ヒストンの改変の特定の変化を含む証拠です.
結論:
- 新しく特定された遺伝子は,冬の寒さの持続時間に対する植物の知覚に重要な役割を果たしています.
- ヴァルナライゼーションは,FLCロカスで遺伝性表遺伝子状態を確立し,安定した抑圧を保証します.
- ヒストンの改変は,FLCのヴァルナライゼーション誘発の静止を媒介する重要な分子イベントです.
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