デンドライトの発達は,カルシウム調節された転写活性化剤であるCRESTによって調節されます
Hiroyuki Aizawa1, Shu-Ching Hu, Kathryn Bobb
1Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
Science (New York, N.Y.)
|January 13, 2004
まとめ
カルシウム反応性トランザクティベーター (CREST) は,脳の発達に不可欠です. それは遺伝子発現と神経細胞の成長を調節し,頂上遺伝子の障害はデンドライト発達の欠陥を引き起こす.
科学分野:
- 神経科学は神経科学である.
- 分子生物学は分子生物学である.
- 発達生物学 発達生物学について
背景:
- 神経細胞の活動は,カルシウム依存性遺伝子発現を通じて,脳の発達に大きな影響を与えます.
- ニューロン活動と発達変化を結びつける分子メカニズムを理解することは不可欠です.
研究 の 目的:
- 発達中の脳におけるカルシウム依存遺伝子の発現を媒介する新しい要因を特定し,特徴づけること.
- ニューロンの発達におけるカルシウム反応性トランザクティベータCRESTの役割を調査する.
主な方法:
- カルシウム反応性トランザクティバータをクローンするためにトランザクティバータトラップ戦略を使用しました.
- クレスト遺伝子に標的を絞った破壊を施したマウスを生成し,分析した.
- 野生型および変異性マウスの皮質ニューロンにおける dendritic 発達を調べた.
主要な成果:
- 新しいカルシウム反応トランザクティベータ,SYTに関連する核タンパク質,CRESTをクローンに成功しました.
- CRESTは,cAMP応答要素結合タンパク質 (CREB) -結合タンパク質 (CBP) と相互作用し,発達中の脳で発現する.
- 機能的なクレスト遺伝子が欠けているマウスは,皮質と海馬のデンドライト発達の障害を示し,皮質ニューロンのカルシウム依存型デンドライトの成長が損なわれています.
結論:
- CREST媒介の転写のカルシウム活性化は,神経細胞形態変異の重要な調節因子である.
- CRESTは,脳の発達中のニューロンの構造的発達において重要な役割を果たします.
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