Sus1は,SAGAヒストンアセチラゼ複合体の機能成分であり,核孔に関連したmRNAの輸出機構である

Susana Rodríguez-Navarro¹, Tamás Fischer, Ming-Juan Luo

¹Biochemie-Zentrum der Universität Heidelberg (BZH), Im Neuenheimer Feld 328, D-69120 Heidelberg, Germany.

Cell

|January 14, 2004

まとめ

Sus1タンパク質は遺伝子転写とmRNAエクスポートとリンクしています. この核タンパク質は,転写および輸出複合体と相互作用し,遺伝子調節とRNA輸送に影響を与えます.

科学分野:

分子生物学は分子生物学である.
細胞生物学細胞生物学
遺伝学遺伝学とは

背景:

遺伝子発現には,核の転写と細胞質の翻訳が含まれています.
mRNAの輸出は,核から細胞質への遺伝情報の転送を容易にする.
核孔複合体は,核と細胞質の間の輸送を調節する.

研究の目的:

トランスクリプションとmRNAエクスポートの両方に関与するタンパク質を特定するために.
新型タンパク質Sus1の遺伝子発現における機能を明らかにする.
転写調節とmRNAエクスポート経路の関係を調査する.

主な方法:

タンパク質の識別と特徴付け.
生化学的相互作用の研究 (例えば共免疫降水).
遺伝子発現プロファイリングのためのDNAマクロアレイ分析.
クロマチン免疫プレシピテーション (ChIP) アッセイ.
変異株におけるmRNA輸出の分析.

主要な成果:

Sus1は核の毛穴に局所された核タンパク質です.
Sus1はSAGA転写複合体とSac3-Thp1mRNA輸出複合体と相互作用する.
Sus1は遺伝子転写の調節に不可欠です.
Sus1は効率的なmRNAエクスポートに必須です.
Sus1は活性化時にSAGA依存遺伝子プロモーターと結合する.

結論:

Sus1はSAGA転写機構とmRNA輸出経路の間の重要なリンクとして機能します.
発見は,転写の開始とmRNAの輸出の間の予期せぬ機能的関連性を明らかにした.
Sus1は,転写からエクスポートまでの遺伝子発現の調整において二重の役割を果たします.

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