ゴルギの膜は,哺乳類の細胞のミトーシス中に,エンドプラズマの網膜から分離され続けます
Matt Yasuo Pecot1, Vivek Malhotra
1Cell and Developmental Biology Department, University of California San Diego, La Jolla, CA 92093, USA.
Cell
|January 14, 2004
まとめ
ゴルギ膜は,哺乳類の細胞におけるミトーシス過程で,エンドプラズマ網膜 (ER) と融合しない. この研究では,新しいラパミシン誘発近接測定法を使用して,ゴルギタンパク質を追跡し,細胞分裂を通してERと異なるままであることを発見しました.
科学分野:
- 細胞生物学 細胞生物学
- 分子生物学は分子生物学である.
- オルガネルダイナミクス オルガネルダイナミクス
背景:
- ミトーシス中の臓器細胞の運命は,細胞分裂に不可欠です.
- ミトーシス中のゴルギとエンドプラズマ網膜 (ER) 膜の関係は不明である.
- 臓器細胞の分離を理解することで,ミトーシス後の適切な細胞機能を確保できます.
研究 の 目的:
- ゲルジ膜がミトーシス過程でERと融合するかどうかを調査する.
- 細胞分裂中にゴルギタンパク質がERタンパク質と相互作用するかどうかを判断する.
- 哺乳類の細胞ミトーシス中のゴルギとERの分離メカニズムを解明する.
主な方法:
- COS細胞におけるFKBPタグされたGolgiタンパク質とERで保持されたFRAPタンパク質を用いた近接分析を開発した.
- FKBPとFRAPとの関連を誘発するためにラパミシンを使用し,相互作用した場合にタンパク質をトラップします.
- ラパミシンの存在下でミトーシス後のゴルギタンパク質の局所化をモニターした.
主要な成果:
- ラパミシン治療は,FKBPと融合したゴルギタンパク質がERに留まることを引き起こしませんでした.
- Golgiタンパク質は,ERではなく,新しく形成されたGolgiスタックで一貫して発見されました.
- ゲルジ膜がミトーシス中にERから物理的に分離していることを示した.
結論:
- ゴルギ膜は,哺乳類の細胞におけるミトーシス全体を通して,ERからの独特のアイデンティティを維持する.
- この研究は,ミトーシス中のゴルギ-ER融合に対する直接的な証拠を提供します.
- 細胞分裂中の臓器細胞膜の相互作用を研究するための信頼できる方法を確立した.
関連する概念動画
01:49Golgi Apparatus
As they leave the Endoplasmic Reticulum (ER), properly folded and assembled proteins are selectively packaged into vesicles. These vesicles are transported by microtubule-based motor proteins and fuse together to form vesicular tubular clusters, subsequently arriving at the Golgi apparatus, a eukaryotic endomembrane organelle that often has a distinctive ribbon-like appearance.The Golgi apparatus is a major sorting and dispatch station for the products of the ER. Newly arriving vesicles enter...
01:09Golgi Apparatus
Properly folded and assembled proteins are selectively packaged into vesicles that exit the ER. Motor proteins transport these vesicles to the Golgi apparatus for adding modifications that make these proteins functional at their destination.
The Golgi apparatus is a eukaryotic organelle that has a distinctive ribbon-like appearance. It is a primary sorting and dispatch station for cargo arriving from the ER. Newly arriving vesicles enter the cis face of the Golgi, closest to the ER, and are...
The Golgi apparatus is a eukaryotic organelle that has a distinctive ribbon-like appearance. It is a primary sorting and dispatch station for cargo arriving from the ER. Newly arriving vesicles enter the cis face of the Golgi, closest to the ER, and are...
01:12Golgi Matrix Proteins
Golgi matrix proteins are a group of highly dynamic proteins that maintain the stacked structure of Golgi. These proteins adapt to rapid morphological changes of the Golgi during the cell cycle. During cell division, mild proteolysis removes these connections resulting in Golgi unstacking. In The daughter cells, these proteins help reassemble the unstacked Golgi.
One of the first identified Golgi matrix proteins was GM130, a rod-like protein located in the cis-Golgi. Subsequently, many Golgi...
One of the first identified Golgi matrix proteins was GM130, a rod-like protein located in the cis-Golgi. Subsequently, many Golgi...
02:33Distribution of Cytoplasmic Content
Cytokinesis segregates a cell’s chromosomes and organelles into its daughter cells. Organelles divide and grow prior to cell division but cannot be synthesized de novo; therefore, cells must receive at least one copy of each organelle to survive. Currently, many of the details of how the organelles are distributed are not yet fully elucidated.
Distribution of cytoplasmic determinants
The cytoplasm contains various organelles, as well as salts, proteins, and water. The distribution of small...
Distribution of cytoplasmic determinants
The cytoplasm contains various organelles, as well as salts, proteins, and water. The distribution of small...
01:26Transport Across the Golgi
While it is unclear how molecules move between adjacent Golgi cisternae, it is apparent that the molecules move from cis- cisterna, the entry face, to the trans- cisterna, the exit face. Experiments initially suggested vesicles that bud from one cisterna and fuse with the next cisterna to transport proteins between the cisternae. This vesicular transport model describes the Golgi apparatus as a relatively static structure with a unique enzyme composition in each cisterna. Molecules are...
01:09Golgi Apparatus
Properly folded and assembled proteins are selectively packaged into vesicles that exit the ER. Motor proteins transport these vesicles to the Golgi apparatus for adding modifications that make these proteins functional at their destination.
The Golgi apparatus is a eukaryotic organelle that has a distinctive ribbon-like appearance. It is a primary sorting and dispatch station for cargo arriving from the ER. Newly arriving vesicles enter the cis face of the Golgi, closest to the ER, and are...
The Golgi apparatus is a eukaryotic organelle that has a distinctive ribbon-like appearance. It is a primary sorting and dispatch station for cargo arriving from the ER. Newly arriving vesicles enter the cis face of the Golgi, closest to the ER, and are...