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Nox4は,内皮NAD(P) H酸化酵素の主要な触媒成分として使用されています
Tetsuro Ago1, Takanari Kitazono, Hiroaki Ooboshi
1Department of Medicine and Clinical Science, Graduate School of Medical Sciences, Kyushu University, Maidashi 3-1-1, Higashi-ku, Fukuoka 812-8582, Japan. agou@intmed2.med.kyushu-u.ac.jp
Circulation
|January 14, 2004
まとめ
Nox4は,血管内皮細胞における活性酸素種の主な源であり,血管疾患に寄与する. この研究では,Nox4が内皮内部のNAD(P) H酸化酵素の主要な成分として特定されています.
科学分野:
- 血管生物学 血管生物学
- 細胞シグナル伝達 細胞信号伝達
- バイオケミストリー バイオケミストリー
背景:
- 反応性酸素種 (ROS) は,血管細胞における重要なシグナル伝達分子です.
- NAD(P) H酸化酵素は,血管内皮細胞におけるROSの主な源である.
- この酸化酵素の正確な構造と活性化は不明である.
研究 の 目的:
- 内皮NAD(P) H酸化酵素の分子同一性と超酸化物生成活性について調べる.
- 内皮のROS生成におけるNox4の役割を決定する.
主な方法:
- 内皮細胞でNox4を表現し,他のNoxタンパク質と比較した.
- 電子スピン共振と化学発光を用いた超酸化物生成の測定.
- アンチセンスオリゴヌクレオチドを用いてダウン調節されたNox4は,そのインビヴォおよびインビトロ効果を評価する.
主要な成果:
- Nox4は,内皮細胞で高度に発現し,gp91phox/Nox2.2のような他のNoxタンパク質を上回りました.
- 内皮膜は,NAD(P) Hに依存したスーパーオキシドの生産を著しく示した.
- Nox4のダウンレギュレーションにより,スーパーオキシドの生産が減少し,その役割が確認されました.
結論:
- Nox4は,内皮NAD・P) H酸化酵素の主要な触媒成分である可能性が高い.
- Nox4は,血管内皮細胞におけるROS生成に重要な役割を果たします.
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