関連する実験動画
Updated: Jul 16, 2026

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Spatiotemporal Analysis of Cytokinetic Events in Fission Yeast
Published on: February 20, 2017
イーストにおけるエンドサイトーシス:小さなGTP結合タンパク質 (Ypt7p) が関与している証拠
H Wichmann1, L Hengst, D Gallwitz
1Max Planck Institute for Biophysical Chemistry, Department of Molecular Genetics, Germany.
Cell
|December 24, 1992
まとめ
イーストのYPT7遺伝子は,真空タンパク質輸送に不可欠なGTP結合タンパク質をコードする. その欠如は,断片化された真空孔とフェロモン分解の障害を引き起こし,エンドソーム輸送における役割を示唆しています.
科学分野:
- 分子生物学は分子生物学である.
- 細胞生物学 細胞生物学
- イースト遺伝学 イースト遺伝学
背景:
- ラス関連タンパク質のYptファミリーは,細胞内輸送に関与しています.
- Rab7はYptタンパク質の哺乳類の同型であり,エンドソーマの密輸に関与しています.
研究 の 目的:
- サッカロマイセス・セレヴィシアのYPT7遺伝子をクローンし,特徴づけること.
- 酵母細胞のプロセス,特に真空輸送におけるYpt7タンパク質の機能を調査する.
主な方法:
- 遺伝子クローニングとS. cerevisiaeの破壊.
- バクオラー形態とタンパク質輸送の分析.
- アルファファクターフェロモンの吸収と分解の測定.
主要な成果:
- クローン化されたYPT7遺伝子は,哺乳類のRab7.7に同質な208アミノ酸のGTP結合タンパク質をコードする.
- YPT7遺伝子の破壊は細胞成長に影響を及ぼさなかったが,断片化された真空穴を生じた.
- ミュータントは,真空タンパク質成熟の欠陥を示し,内細胞化フェロモンの分解をひどく抑制しました.
結論:
- Ypt7pは酵母におけるエンドソームのような区間間のタンパク質輸送に重要な役割を果たしています.
- この発見は,Ypt/Rabタンパク質が,膀性トラフィックの経路における保存された機能を強調しています.
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