ネズミのサイトメガロウイルス感染症は,アテロスクレロティック性遺伝子の大動脈発現を増加させる
Mary Susan Burnett1, Sarfraz Durrani, Eugenio Stabile
1Cardiovascular Research Institute, MedStar Research Institute, Washington Hospital Center, Washington, DC 20010, USA. mary.s.burnett-miller@medstar.net
Circulation
|February 6, 2004
まとめ
マウリン・サイトメガロウイルス (MCMV) 感染症は,アテロスクレロティック病変のサイズとマウスのプロアテロゲン遺伝子発現を増加させます. これらの発見は,心血管疾患の発症および進行におけるサイトメガロウイルスの役割を示唆しています.
科学分野:
- 心血管疾患に関する研究
- 感染症免疫学 感染症免疫学
- 動脈硬化症の病原性 病原性
背景:
- 心血管疾患における感染症の役割については,依然として議論されている.
- 以前の研究では,ネズミのサイトメガロウイルス (MCMV) 感染がアポリポプロテインE欠乏症 (apoE-/-) のマウスで動脈硬化症を悪化させることが示されました.
- この研究では,MCMVが大動脈の遺伝子発現と病変の発生に及ぼす影響を調査しています.
研究 の 目的:
- MCMV感染が,大動脈遺伝子発現におけるプロアテロゲン性変化を誘発するかどうかを判断する.
- コレステロールを摂取したマウスの大動脈損傷領域に対するMCMV感染の影響を調べる.
主な方法:
- マウス (apoE-/およびC57BL/6J) はMCMVに感染した.
- 大動脈の遺伝子発現は,RNA抽出,アフィメトリックスチップ,TaqMan RT-PCRを用いて分析された.
- 大動脈シヌス病変の領域は,コレステロールを摂取したマウスのオイルレッドO染色を用いて評価されました.
主要な成果:
- MCMV感染は,若いマウスの主要なアテロゲン遺伝子 (MCP-1,MIG,IP-10) の大動脈発現を変化させた.
- 感染は,既存の病変を有する高齢のapoE-/-マウスのMCP-1発現を増加させた.
- 動脈硬化病変の領域は,MCMV感染後のコレステロールを摂取したマウスで有意に増加しました.
結論:
- MCMV感染は,動脈硬化病変の領域と動脈原遺伝子の発現を明らかに増加させる.
- これらの発見は,サイトメガロウイルスが動脈硬化症の発症および進行に寄与する潜在的なメカニズムを明らかにします.
- この結果は,感染が動脈硬化疾患において重要な役割を果たすという仮説を裏付けている.
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