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Updated: Jul 16, 2026
07:59
Folding and Characterization of a Bio-responsive Robot from DNA Origami
Published on: December 3, 2015
1.7キロ塩基単一鎖のDNAが,ナノスケールオクタエドロンに折りたたまれています
William M Shih1, Joel D Quispe, Gerald F Joyce
1Department of Chemistry and The Skaggs Institute for Chemical Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, USA. wmshih@scripps.edu
Nature
|February 13, 2004
まとめ
研究者らは,自己組み立て可能なDNA八面体を作るための新しいDNAオリガミ技術を設計した. この方法は,複雑なDNAナノ構造のスケーラブルな生産を可能にし,各コンポーネントのユニークなアドレス性を可能にします.
科学分野:
- ナノテクノロジー ナノテクノロジー
- 分子生物学は分子生物学である.
- バイオフィジックス 生物物理学
背景:
- 分子自己組み立ては,ナノ構造物を構築するために特定のDNA塩基配列を使用します.
- 既存の3DDNAオブジェクトには,ポリメラーゼベースのクローンに適したトポロジーがなく,大規模生産を制限しています.
- スケーラブルな生産は,DNAナノテクノロジーアプリケーションの進歩に不可欠です.
研究 の 目的:
- 3Dの幾何学的なDNAオブジェクトを作成するためにポリメラーゼ増幅に対応するDNA分子を設計および合成する.
- このDNA分子を安定した,複雑なナノ構造に折りたたむ方法を開発する.
- DNAナノ構造内の個々のコンポーネントのユニークなアドレッサビリティを可能にするために.
主な方法:
- 1,669ヌクレオチド単鎖DNA分子の設計と合成.
- 5つの合成オリゴデオキシヌクレオチドを用いてDNA分子をオクターヘッド構造に折り畳む.
- 折りたたまれたDNAオクタヘッドの構造的特徴化のための冷凍電子顕微鏡.
主要な成果:
- 約22ナノメートルの直径の空洞の八面体へとDNA分子が折り畳まれる.
- 八面体構造は,六つの四方向の接点で結合された12のストラットで構成されています.
- 各ストラットにはユニークなベースペア配列があり,個々のアドレッシングが可能になります.
結論:
- 複雑な3DDNAナノ構造を構築するために,新しい,増幅可能なDNAオリガミ方法が開発されました.
- 合成されたDNAオクタヘッドは,冷凍電子顕微鏡で構造的に検証されています.
- 個々のストラットのユニークなシーケンスアドレッシビリティは,高度な分子プログラミングとナノスケールデバイス製造の可能性を開きます.
関連する概念動画
01:16The DNA Helix
Overview
01:16The DNA Helix
Overview
02:21Chromatin Packaging
Each human somatic cell contains 6 billion base-pairs of DNA. Each base-pair is 0.34 nm long, which means that each diploid cell contains a staggering 2 meters of DNA. How is such a long DNA strand packed inside a nucleus measuring only 10 - 20 microns in diameter?
The chromatin
In combination with specialized DNA binding protein called Histones, the DNA double helix forms a compact DNA: protein complex called chromatin. The chromatin itself is further compacted into higher-order structures.
The chromatin
In combination with specialized DNA binding protein called Histones, the DNA double helix forms a compact DNA: protein complex called chromatin. The chromatin itself is further compacted into higher-order structures.
01:07The DNA Helix
Deoxyribonucleic acid, or DNA, is the genetic material responsible for passing traits from generation to generation in all organisms and most viruses. DNA is composed of two strands of nucleotides that wind around each other to form a spring-like structure called a double helix. However, the double helix is not perfectly symmetrical. Instead, there are regularly occurring grooves in the structure. The major groove occurs where the sugar-phosphate backbones are relatively far apart. This space...
01:19The Nucleosome
Human DNA is almost two meters long. However, it is compressed inside a tiny nucleus measuring only a few microns in diameter. To make this degree of compaction possible, DNA is organized into several sequential levels so that it can fit into such a tiny space. The most compact form of DNA is a chromosome that can be seen under a microscope in a dividing cell.
In a chromosome, DNA is wound twice around a protein complex called a histone octamer core, which consists of 8 histone proteins. This...
In a chromosome, DNA is wound twice around a protein complex called a histone octamer core, which consists of 8 histone proteins. This...
01:32Chromatin Packaging
Each human somatic cell contains 6 billion base pairs of DNA. Each base pair is 0.34 nm long, meaning each diploid cell contains a staggering 2 meters of DNA. This long DNA strand is packed inside a nucleus measuring only 10-20 microns in diameter with the help of specialized DNA-binding proteins called histones. Together they form a compact DNA-protein complex called chromatin. The chromatin is further compacted into higher-order structures. The highest level of compaction is achieved during...