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Updated: Jul 10, 2026

10:19
Derivation of Mouse Trophoblast Stem Cells from Blastocysts
Published on: June 8, 2010
撤回: 複製された芽細胞から派生した多能性のヒト胚性幹細胞系統の証拠
Woo Suk Hwang1, Young June Ryu, Jong Hyuk Park
1College of Veterinary Medicine, Seoul National University, Seoul 151-742, Korea. hwangws@snu.ac.kr
まとめ
研究者らは,体細胞核移転 (SCNT) を用いてクローンされた芽細胞からヒト胚性幹細胞 (ES) 細胞系を導いた. これらのSCNT-ヒトES細胞は多能であり,遺伝的にドナーと同一であり,SCNTをヒトES細胞生成の有効な方法として支持しています.
科学分野:
- 生殖生物学 生殖生物学
- 幹細胞科学 幹細胞科学とは
- 遺伝学 遺伝学とは
背景:
- ソマティック細胞核移転 (SCNT) は,遺伝的に同一の動物の創造を可能にします.
- 人間の胚性幹細胞 (ES細胞) を得ることは,再生医療と疾患モデリングにおいて極めて重要です.
研究 の 目的:
- SCNTを用いてクローン化されたヒト芽細胞から,多能性のヒト胚性幹細胞系を導出,特徴づけること.
- SCNTの起源を確認し,派生細胞の多能性と遺伝的安定性を評価する.
主な方法:
- ソマティック細胞核移転 (SCNT) は,ヒト卵細胞で実施されました.
- 派生ブレストシストは,胚性幹細胞系を確立するために使用されました.
- 特徴付けには,形質学,細胞表面マーカー,インビトロ分化 (胚体),インビボテラトマ形成,カリオタイプ化,インプリント分析などが含まれていた.
主要な成果:
- クローンされた芽細胞から,多能性のヒトES細胞系 (SCNT-hES-1) が成功裏に導出されました.
- SCNT-hES-1細胞は,多能性と3つの生殖層全体への分化能力を含む,典型的なES細胞の特徴を示した.
- 細胞は正常なカリオタイプを維持し,70通路以上でドナー細胞との遺伝的同一性を維持しました.
- インプリント分析はSCNTの起源を支持したが,パートノゲネティック起源は完全に排除できなかった.
結論:
- SCNTは,多能性のヒトES細胞を生成するための実現可能な方法です.
- 派生したSCNT-hES-1細胞系は遺伝的に安定しており,多能性を保持しています.
- これらの発見は,治療用クローニングと幹細胞研究に意味を持つ.
関連する概念動画
Reproductive Cloning
Reproductive cloning is the process of producing a genetically identical copy—a clone—of an entire organism. While clones can be produced by splitting an early embryo—similar to what happens naturally with identical twins—cloning of adult animals is usually done by a process called somatic cell nuclear transfer (SCNT).
Somatic Cell Nuclear Transfer
In SCNT, an egg cell is taken from an animal and its nucleus is removed, creating an enucleated egg. Then a somatic cell—any cell that is not a sex...
Somatic Cell Nuclear Transfer
In SCNT, an egg cell is taken from an animal and its nucleus is removed, creating an enucleated egg. Then a somatic cell—any cell that is not a sex...
Fertilization
During fertilization, an egg and sperm cell fuse to create a new diploid structure. In humans, the process occurs once the egg has been released from the ovary, and travels into the fallopian tubes. The process requires several key steps: 1) sperm present in the genital tract must locate the egg; 2) once there, sperm need to release enzymes to help them burrow through the protective zona pellucida of the egg; and 3) the membranes of a single sperm cell and egg must fuse, with the sperm...
Cleavage and Blastulation
After a large-single-celled zygote is produced via fertilization, the process of cleavage occurs while zygotes travel through the uterine tube. Cleavage is a mitotic cell division that does not result in growth. With each round of successive cell division, daughter cells get increasingly smaller.
Reproductive Cloning
Reproductive cloning is the process of producing a genetically identical copy—a clone—of an entire organism. While clones can be produced by splitting an early embryo—similar to what happens naturally with identical twins—cloning of adult animals is usually done by a process called somatic cell nuclear transfer (SCNT).
Somatic Cell Nuclear Transfer
In SCNT, an egg cell is taken from an animal and its nucleus is removed, creating an enucleated egg. Then a somatic cell—any cell that is not a sex...
Somatic Cell Nuclear Transfer
In SCNT, an egg cell is taken from an animal and its nucleus is removed, creating an enucleated egg. Then a somatic cell—any cell that is not a sex...
Zygotic Development And Stem Cell Formation
The development of all multicellular organisms starts with the fusion of haploid cells called sperm and egg to form a diploid zygote. A zygote is a totipotent cell that can develop into a complete organism. The zygote undergoes cell division or cleavage to form an 8-cell mass. Until this stage, the cells are spherical, loosely attached, and remain totipotent. Totipotent cells are capable of developing both the embryonic and the extraembryonic tissues. However, as they continue to divide, they...
Maintenance of the ES Cell State
The cells of the blastocyst inner cell mass only remain pluripotent for a short time. This state of pluripotency and self-renewal can be maintained in embryonic stem (ES) cell culture by adding specific chemicals or growth factors to ensure the cells can continue dividing and later differentiate into different cell types. In some cases, the cells are grown on a feeder layer of differentiated cells, which provides the growth factors and extracellular matrix components necessary for stem cell...

