TFIIH転写因子,リフトバレー出血熱ウイルスの標的である
Nicolas Le May1, Sandy Dubaele, Luca Proietti De Santis
1Unité de Génétique Moléculaire des Bunyaviridés, Institut Pasteur, 25 rue du Dr Roux, 75724 Paris, Cedex 15, France.
Cell
|February 26, 2004
まとめ
リフトバレー熱ウイルス (RVFV) は,TFIIH転写因子を標的にして宿主RNA合成を抑制する. ウイルスのNSsタンパク質はTFIIHの組み立てを妨害し,宿主細胞の不安定化と免疫回避につながります.
科学分野:
- ウイルス学 ウイルス学 ウイルス学
- 分子生物学は分子生物学である.
- 細胞生物学 細胞生物学
背景:
- リフトバレー熱ウイルス (RVFV) は,人間や動物に重度の病気を引き起こす.
- RVFV感染は,宿主細胞のRNA合成をひどく阻害する.
研究 の 目的:
- RVFVが宿主RNA合成を抑制する分子メカニズムを解明する.
- ホストの転写を妨害するウイルスタンパク質を特定する.
主な方法:
- イースト2ハイブリッドシステムです.
- 再結合タンパク質の発現と浄化.
- コンフォカル顕微鏡によるコンフォカル顕微鏡検査
主要な成果:
- RVFV感染は,転写因子TFIIHの濃度を低下させる.
- ウイルスの非構造的NSsタンパク質は,TFIIHサブユニットp44とXPBと相互作用する.
- NSsタンパク質は核繊維を形成し,TFIIHの成分を隔離し,その組み立てを防ぐ.
- この障害は,XPD結合機能と競合し,TFIIH機能を不安定化します.
結論:
- RVFVはNSsタンパク質を使用して,TFIIHアセンブリを干渉することによって宿主転写を妨害します.
- このメカニズムは,宿主の免疫反応を回避するRVFVの能力にとって極めて重要です.
- TFIIHを標的にすることは,複製と病原性の重要なウイルス戦略です.
関連する概念動画
01:31Translation
Lesson: Translation
Translation is the process of synthesizing proteins from the genetic information carried by messenger RNA (mRNA). Following transcription, it constitutes the final step in the expression of genes. This process is carried out by ribosomes, complexes of protein and specialized RNA molecules. Ribosomes, transfer RNA (tRNA), and other proteins produce a chain of amino acids—the polypeptide—as the end product of translation.
Translation Produces the Building Blocks of Life
Translation is the process of synthesizing proteins from the genetic information carried by messenger RNA (mRNA). Following transcription, it constitutes the final step in the expression of genes. This process is carried out by ribosomes, complexes of protein and specialized RNA molecules. Ribosomes, transfer RNA (tRNA), and other proteins produce a chain of amino acids—the polypeptide—as the end product of translation.
Translation Produces the Building Blocks of Life
02:16Transcription Factors
Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
02:16Transcription Factors
Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
02:28Leaky Scanning
During most eukaryotic translation processes, the small 40S ribosome subunit scans an mRNA from its 5' end until it encounters the first start AUG codon. The large 60S ribosomal subunit then joins the smaller one to initiate protein synthesis. The location of the translation initiation is largely determined by the nucleotides near the start codon as there may be multiple translation initiation sites present on the mRNA. Marilyn Kozak discovered that the sequence RCCAUGG (where R stands for...
01:30General Transcription Factors
Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
01:31Translation
Lesson: Translation
Translation is the process of synthesizing proteins from the genetic information carried by messenger RNA (mRNA). Following transcription, it constitutes the final step in the expression of genes. This process is carried out by ribosomes, complexes of protein and specialized RNA molecules. Ribosomes, transfer RNA (tRNA), and other proteins produce a chain of amino acids—the polypeptide—as the end product of translation.
Translation Produces the Building Blocks of Life
Translation is the process of synthesizing proteins from the genetic information carried by messenger RNA (mRNA). Following transcription, it constitutes the final step in the expression of genes. This process is carried out by ribosomes, complexes of protein and specialized RNA molecules. Ribosomes, transfer RNA (tRNA), and other proteins produce a chain of amino acids—the polypeptide—as the end product of translation.
Translation Produces the Building Blocks of Life