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Lignin Down-regulation of Zea mays via dsRNAi and Klason Lignin Analysis
Published on: July 23, 2014
ロールされた葉1のマイクロRNA媒介による抑圧は,トウモロコシ葉の極性を指定します
Michelle T Juarez1, Jonathan S Kui, Julie Thomas
1Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 11724, USA.
Nature
|March 6, 2004
まとめ
マイクロRNA (miRNA) は,発達信号として作用する. MicroRNA166は,植物におけるHD-ZIPIII遺伝子発現を調節することによって,葉の極性を定義する保存された偏振信号です.
科学分野:
- 植物発達生物学 植物発達生物学
- 分子遺伝学 分子遺伝学
- マイクロRNA機能 マイクロRNA機能
背景:
- 植物の葉の発達には,軸の仕様が含まれています.
- マイクロRNA (miRNA) は,発達に関与する調節分子である.
- クラスIIIホメオドメイン/ルシンジッパー (HD-ZIPIII) 遺伝子は,アダキシアル葉の運命を決定する.
研究 の 目的:
- 植物葉の極性におけるmiRNA166の役割を調査する.
- miRNA166が偏振信号として作用するかどうかを判断する.
- 葉の発達中のmiRNA166の空間的および時間的な発現を理解する.
主な方法:
- トウモロコシのmiRNA166発現パターンの分析.
- miRNA166とHD-ZIPIII遺伝子の相互作用を調査する.
- イン・ビトロ・クリバージ・アッセイ.
主要な成果:
- マイクロRNA166発現パターンは,トウモロコシ hd-zipIII遺伝子,ロールされた葉1 (rld1) の発現領域を空間的に定義します.
- miRNA166の発現は葉の発達中に徐々に拡大する.
- miRNA166はフロームに蓄積し,それは移動信号である可能性があることを示唆しています.
結論:
- MicroRNA166は,葉のアダキシアル/アバキシアルアイデンティティを確立するために不可欠な保存された偏振信号です.
- miRNA166は,シグナリングセンターから発信される移動信号分子として機能する可能性があります.
- miRNA166の機能を理解することで,植物の発達過程の洞察が得られます.
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MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...

