非肺性SARSコロナウイルス感染症とSARSコロナウイルス肺炎の相対的な割合
Patrick C Y Woo1, Susanna K P Lau, Hoi-wah Tsoi
1Department of Microbiology, University of Hong Kong, University Pathology Building, Queen Mary Hospital, Hong Kong.
Lancet (London, England)
|March 20, 2004
まとめ
サブクリニカル重症急性呼吸器症候群コロナウイルス (SARS-CoV) 感染症は,肺炎症例よりも一般的です. この研究では,SARS-CoV抗体を検出する高度に敏感で特定のELISAテストを開発し,広範で検出されていない感染を明らかにしました.
科学分野:
- ウイルス学 ウイルス学 ウイルス学
- 免疫学 免疫学とは
- エピデミオロジー エピデミオロジー
背景:
- 重症急性呼吸器症候群コロナウイルス (SARS-CoV) のゲノムが知られているが,血清発生率とサブクリニック感染のスクリーニングが不足している.
- サブクリニックおよび非肺性SARS-CoV感染を特定することは,病気の伝播を理解するために重要である.
研究 の 目的:
- サルス-CoV感染の血清流行度を評価するために,サブクリニックおよび非肺炎症例を含む.
- SARS-CoV抗体に対する信頼性の高い診断検査を開発し,検証する.
主な方法:
- 免疫性試験のためのクローン・浄化されたSARS-CoV核カプシドタンパク質とスパイクポリペプチド.
- 複合核カプシドタンパク質を用いたIgG抗体ELISAを開発し,ウエスタンブロットで検証しました.
- 血液提供者,非肺炎入院患者,医療従事者のSARS-CoV抗体検診を行いました.
主要な成果:
- SARS-CoVの核カプシドとスパイクタンパク質は,高度に免疫原性であった.
- 開発されたELISAは100%の特異性と94.3%の感度を示した.
- 研究集団の0.48%でIgG抗体が検出され,サブクリニック感染を示唆しています.
結論:
- 発見は,亜臨床的または非肺性SARS-CoV感染の存在を支持しています.
- サブクリニックのSARS-CoV感染症は,研究対象地域ではSARS-CoV肺炎よりもより一般的であることが判明しました.
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