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Characterization of Anisotropic Leaky Mode Modulators for Holovideo
Published on: March 19, 2016
フォトトロピンのLOV2ドメイン:可逆光色スイッチ
John T M Kennis1, Ivo H M van Stokkum, Sean Crosson
1Department of Biophysics, Faculty of Sciences, Vrije Universiteit, 1081HV Amsterdam, The Netherlands. j.kennis@few.vu.nl
Journal of the American Chemical Society
|April 9, 2004
まとめ
光,酸素,または電圧 (LOV) ドメインは,青い光に敏感なタンパク質です. 超高速スペクトロスコーピーは,LOV2ドメインが,青い光で活性化され,近紫外線で無効化される可逆光染色スイッチとして作用することを明らかにしています.
科学分野:
- 写真生物学 写真生物学
- タンパク質科学 タンパク質科学
- バイオフィジックス 生物物理学
背景:
- 光,酸素,または電圧 (LOV) ドメインは青光光受容体である.
- 彼らは光の感受性のためにフラビン染色体を使用します.
- LOV2ドメインは,青光吸収時に共性アドクトを形成します.
研究 の 目的:
- LOV2ドメインの共性アドクト状態の光反応性を調査する.
- アダクト・ボンド改革のメカニズムとスケールを決定する.
- LOV2ドメインのフォトクロミックスイッチの動作を確立するために.
主な方法:
- 超高速スペクトロスコピーは,LOV2.2の光学的に蓄積された共振付添加物に適用されました.
- アドクト状態による近紫外線光子の吸収が研究されました.
- 協和結合ダイナミクスの時間解析分析.
主要な成果:
- 近紫外線光の吸収により,超高速 (100 ps) の共振結合の破裂が引き起こされます.
- 青い光に敏感な基本状態は急速に回復します.
- LOV2領域における可逆光色交換の実証.
結論:
- LOV2ドメインは,可逆光色スイッチとして機能します.
- 青い光はコヴァレンントアドクトを形成することによってスイッチを活性化します.
- 近紫外線 (near-UV) の光は,アドクト結合を断ち切ってスイッチを無効化する.
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