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Cooperative Binding of Transcription Regulators02:13

Cooperative Binding of Transcription Regulators

Transcriptional regulators bind to specific cis-regulatory sequences in the DNA to regulate gene transcription. These cis-regulatory sequences are very short, usually less than ten nucleotide pairs in length. The short length means that there is a high probability of the exact same sequence randomly occurring throughout the genome.  Since regulators can also bind to groups of similar sequences, this further increases the chances of random binding. Transcriptional regulators form dimers that...
Single-Strand DNA Binding Proteins01:03

Single-Strand DNA Binding Proteins

For successful DNA replication, the unwinding of double-stranded DNA must be accompanied by stabilization and protection of the separated single strands of the DNA. This crucial task is performed by single-strand DNA-binding (SSB) proteins. They bind to the DNA in a sequence-independent manner, which means that the nitrogenous bases of the DNA need not be present in a specific order for binding of SSB proteins to it. The binding of SSB proteins straightens single-stranded DNA (ssDNA) and makes...
Assembly of Signaling Complexes01:30

Assembly of Signaling Complexes

Multiprotein signaling complexes are formed in a dynamic process involving protein-protein interactions at the cytoplasmic domain of transmembrane receptors or enzymatic and non-enzymatic proteins associated with the receptor. These complexes ensure the activation and propagation of intracellular signals that regulate cell functions.
Interaction domains in cell signaling
Interaction domains recognize exposed features of their binding partners containing post-translationally modified sequences,...
Selectins01:25

Selectins

Cell adhesion is  an essential aspect of multicellularity. While stable cell interactions usually occur between cells of the same type, transient cell interactions occur between cells of different tissue types, such as between neutrophils and endothelial cells. Selectins are one class of cell adhesion molecules (CAMs) that bind carbohydrate ligands to form transient cell adhesion. They are rod-like proteins with a long extracellular part of variable length ending with the lectin domain, which...
Drug-Receptor Bonds01:25

Drug-Receptor Bonds

Drug-receptor bonds are formed through various chemical forces when drugs interact with target cells. Covalent bonds, strong and irreversible, are exemplified by DNA-alkylating anticancer agents that inhibit cell division. However, such irreversible drug binding lacks selectivity and can modify the DNA of the surrounding healthy cells. Covalent binding often contributes to tissue toxicity, as seen with chloroform and paracetamol metabolites binding to the liver, causing hepatotoxicity.
In...
Transducer Mechanism: Enzyme-Linked Receptors01:27

Transducer Mechanism: Enzyme-Linked Receptors

Enzyme-linked receptors are cell-surface receptors acting as an enzyme or associating with an enzyme intracellularly. They make excellent drug targets. Drugs can bind to the extracellular ligand-binding domain or directly affect their enzymatic domain and alter their activity.
Major types that are helpful drug targets include:

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関連する実験動画

Updated: Jul 9, 2026

A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay
12:31

A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay

Published on: February 28, 2015

エストロゲン受容体マイクロアレイ:サブタイプ選択型リガンド結合

Sung Hoon Kim1, Anobel Tamrazi, Kathryn E Carlson

  • 1Department of Chemistry, University of Illinois, 600 South Matthews Avenue, Urbana, Illinois 61801, USA.

Journal of the American Chemical Society
|April 15, 2004
PubMed
まとめ

私たちは,エストロゲン受容体 (ERalpha-LBDとERbeta-LBD) を研究するための新しい核ホルモン受容体マイクロアレイを開発しました. この高通量メソッドは,リガンド結合を効率的に評価し,他の受容体および同調子器の募集に適応することができます.

さらに関連する動画

Detecting the Ligand-binding Domain Dimerization Activity of Estrogen Receptor Alpha Using the Mammalian Two-Hybrid Assay
09:07

Detecting the Ligand-binding Domain Dimerization Activity of Estrogen Receptor Alpha Using the Mammalian Two-Hybrid Assay

Published on: December 19, 2018

Creating Highly Specific Chemically Induced Protein Dimerization Systems by Stepwise Phage Selection of a Combinatorial Single-Domain Antibody Library
10:17

Creating Highly Specific Chemically Induced Protein Dimerization Systems by Stepwise Phage Selection of a Combinatorial Single-Domain Antibody Library

Published on: January 14, 2020

関連する実験動画

Last Updated: Jul 9, 2026

A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay
12:31

A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay

Published on: February 28, 2015

Detecting the Ligand-binding Domain Dimerization Activity of Estrogen Receptor Alpha Using the Mammalian Two-Hybrid Assay
09:07

Detecting the Ligand-binding Domain Dimerization Activity of Estrogen Receptor Alpha Using the Mammalian Two-Hybrid Assay

Published on: December 19, 2018

Creating Highly Specific Chemically Induced Protein Dimerization Systems by Stepwise Phage Selection of a Combinatorial Single-Domain Antibody Library
10:17

Creating Highly Specific Chemically Induced Protein Dimerization Systems by Stepwise Phage Selection of a Combinatorial Single-Domain Antibody Library

Published on: January 14, 2020

科学分野:

  • バイオケミストリー バイオケミストリー
  • 分子生物学は分子生物学である.
  • エンドクリノロジー エンドクリノロジー

背景:

  • 核ホルモン受容体は,細胞のプロセスにおいて重要な役割を果たします.
  • エストロゲン受容体 (ERs) は,ホルモン調節と疾患を理解するための重要な標的である.
  • 受容体-リガンドの相互作用を研究するための高通量メソッドの開発は不可欠です.

研究 の 目的:

  • 初の核ホルモン受容体マイクロアレイを紹介します.
  • エストロゲン受容体アルファとベータ (ERalpha-LBDとERbeta-LBD) のリガンド結合ドメインを用いてその応用を例示します.
  • リガンド結合と亜型選択性の研究におけるその有用性を実証する.

主な方法:

  • タンパク質 (ERalpha-LBDとERbeta-LBD) を印刷してアルデヒドスライドに貼り付けました.
  • 結合効率は,リガンド結合状態 (アゴニスト対アゴニスト/無リガンド) に基づいて分析されました.
  • リガンド結合活性は,競争的な結合アッセイでエストラジオール-フッ素ホルモコンジュガットを使用して評価されました.

主要な成果:

  • スライドへの結合効率は,アゴニストに結合した形態と比較して,結合されていないまたは対抗剤に結合したER-LBDsでは著しく高く,方向性固有の結合を示唆しています.
  • 固定されたERは,重要なリガンド結合活性を維持した.
  • ERalpha-LBDとERbeta-LBDへのリガンドの特異的および亜型選択的結合が成功裏に決定されました.

結論:

  • 新しい,高通量核ホルモン受容体マイクロアレイ技術が確立されました.
  • この方法は,エストロゲン受容体へのリガンド結合の効率的かつ選択的な評価を可能にします.
  • このプラットフォームは,他の核ホルモン受容体とコレギュレータ募集の研究に適応できます.