Jove
Visualize
お問い合わせ
JoVE
x logofacebook logolinkedin logoyoutube logo
JoVEについて
概要リーダーシップブログJoVEヘルプセンター
著者向け
出版プロセス編集委員会範囲と方針査読よくある質問投稿
図書館員向け
推薦の声購読アクセスリソース図書館諮問委員会よくある質問
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experimentsアーカイブ
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教員リソースセンター教員サイト
利用規約
プライバシーポリシー
ポリシー

関連する概念動画

Short-distance Transport of Resources02:12

Short-distance Transport of Resources

Short-distance transport refers to transport that occurs over a distance of just 2-3 cells, crossing the plasma membrane in the process. Small uncharged molecules, such as oxygen, carbon dioxide, and water, can diffuse across the plasma membrane on their own. In contrast, ions and larger molecules require the assistance of transport proteins due to their charge or size. Transport across membranes also occurs within individual cells, playing a variety of essential roles for the plant as a whole.
Vesicular Tubular Clusters01:45

Vesicular Tubular Clusters

After budding out from the ER membrane, some COPII vesicles lose their coat and fuse with one another to form larger vesicles and interconnected tubules called vesicular tubular clusters or VTCs. These clusters constitute a compartment at the ER-Golgi interface known as ERGIC (Endoplasmic Reticulum Golgi Intermediate Compartment). The ERGIC is a mobile membrane-bound cargo transport system that sorts proteins secreted from ER and delivers them to the Golgi.
With the help of motor proteins such...
Spin–Spin Coupling: One-Bond Coupling01:17

Spin–Spin Coupling: One-Bond Coupling

Coupling interactions are strongest between NMR-active nuclei bonded to each other, where spin information can be transmitted directly through the pair of bonding electrons. While nuclei polarize their electrons to the opposite spins, the bonding electron pair has opposite spins. Configurations with antiparallel nuclear spins are expected to be lower in energy. When coupling makes antiparallel states more favorable, J is considered to have a positive value. The one-bond coupling constant, 1J,...
Spin–Spin Coupling: Two-Bond Coupling (Geminal Coupling)01:20

Spin–Spin Coupling: Two-Bond Coupling (Geminal Coupling)

Two NMR-active nuclei bonded to a central atom can be involved in geminal or two-bond coupling. Geminal coupling is commonly seen between diastereotopic protons in chiral molecules and unsymmetrical alkenes, among others.
The central atom need not be NMR-active because its electrons are affected by the electron polarization of the spin-active atoms. However, spin information is transmitted less effectively than in one-bond coupling, and 2J values are usually weaker than 1J values. The energy of...
Spin–Spin Coupling: Three-Bond Coupling (Vicinal Coupling)01:22

Spin–Spin Coupling: Three-Bond Coupling (Vicinal Coupling)

Vicinal or three-bond coupling is commonly observed between protons attached to adjacent carbons. Here, nuclear spin information is primarily transferred via electron spin interactions between adjacent C‑H bond orbitals. This generally favors the antiparallel arrangement of spins, so 3J values are usually positive.
The extent of coupling depends on the C‑C bond length, the two H‑C‑C angles, any electron-withdrawing substituents, and the dihedral angle between the involved orbitals. The...
¹H NMR: Long-Range Coupling01:27

¹H NMR: Long-Range Coupling

The coupling interactions of nuclei across four or more bonds are usually weak, with J values less than 1 Hz. While these are usually not observed in spectra, the presence of multiple bonds along the coupling pathway can result in observable long-range coupling.
In alkenes, spin information is communicated via σ–π overlap, as seen in allylic (four-bond) and homoallylic (five-bond) couplings. These coupling interactions are stronger when the σ bond is parallel to the alkene π orbitals.

こちらも読む

関連記事

共著者、ジャーナル、引用グラフによってこの研究に関連する記事。

並び替え
Same author

Not just Salk.

Science (New York, N.Y.)·2017
Same author

CRISPR germline engineering--the community speaks.

Nature biotechnology·2015
Same author

Biotechnology. A prudent path forward for genomic engineering and germline gene modification.

Science (New York, N.Y.)·2015
Same author

DNA replication: making two forks from one prereplication complex.

Molecular cell·2010
Same author

Single particle EM studies of the Drosophila melanogaster origin recognition complex and evidence for DNA wrapping.

Journal of structural biology·2008
Same author

Cell biology: a switch for S phase.

Nature·2007
Same journal

Whole-cell particle-based digital twin simulations from 4D lattice light-sheet microscopy data.

Cell·2026
Same journal

Systematic discovery of pathogen effector functions across human pathogens and pathways.

Cell·2026
Same journal

Structural basis for host membrane binding and remodeling by invading malaria parasites.

Cell·2026
Same journal

Multiscale integration of tissue and chromatin context converts cell heterogeneity into stable intestinal patterning.

Cell·2026
Same journal

Arc mediates intercellular tau transmission via extracellular vesicles.

Cell·2026
Same journal

Electromagnetic field-inducible in vivo gene switch for remote spatiotemporal control of gene expression.

Cell·2026
関連記事をすべて見る

関連する実験動画

Updated: May 10, 2026

Quantitation and Analysis of the Formation of HO-Endonuclease Stimulated Chromosomal Translocations by Single-Strand Annealing in Saccharomyces cerevisiae
09:40

Quantitation and Analysis of the Formation of HO-Endonuclease Stimulated Chromosomal Translocations by Single-Strand Annealing in Saccharomyces cerevisiae

Published on: September 23, 2011

コバレンツ・インテグレーションなしでオチハイクする.

Michael Botchan1

  • 1Department of Cell and Molecular Biology and Division of Biochemistry and Molecular Biology, The University of California, Berkeley, Berkeley, CA 94720, USA.

Cell
|April 28, 2004
PubMed
まとめ
この要約は機械生成です。

パピローマウイルスなどの潜伏ウイルスは,細胞分裂のために宿主染色体に結合します. この研究は,動物性パピローマウイルスの染色体上の保持に関与する細胞タンパク質を明らかにし,ウイルスの持続性に関する新しい洞察を提供します.

さらに関連する動画

Site-Directed φC31-Mediated Integration and Cassette Exchange in Anopheles Vectors of Malaria
09:38

Site-Directed φC31-Mediated Integration and Cassette Exchange in Anopheles Vectors of Malaria

Published on: February 2, 2021

Covalent Fragment Screening Using the Quantitative Irreversible Tethering Assay
06:17

Covalent Fragment Screening Using the Quantitative Irreversible Tethering Assay

Published on: February 28, 2025

関連する実験動画

Last Updated: May 10, 2026

Quantitation and Analysis of the Formation of HO-Endonuclease Stimulated Chromosomal Translocations by Single-Strand Annealing in Saccharomyces cerevisiae
09:40

Quantitation and Analysis of the Formation of HO-Endonuclease Stimulated Chromosomal Translocations by Single-Strand Annealing in Saccharomyces cerevisiae

Published on: September 23, 2011

Site-Directed φC31-Mediated Integration and Cassette Exchange in Anopheles Vectors of Malaria
09:38

Site-Directed φC31-Mediated Integration and Cassette Exchange in Anopheles Vectors of Malaria

Published on: February 2, 2021

Covalent Fragment Screening Using the Quantitative Irreversible Tethering Assay
06:17

Covalent Fragment Screening Using the Quantitative Irreversible Tethering Assay

Published on: February 28, 2025

科学分野:

  • ウイルス学 ウイルス学 ウイルス学
  • 分子生物学は分子生物学である.
  • 細胞生物学 細胞生物学

背景:

  • 多くの潜伏ウイルスは,真核ミト菌染色体に非共性的に結合する.
  • この結合は,細胞分裂中にウイルスのゲノム分割を保証する.
  • ウイルスの要素は知られているが,この染色体結合を媒介する細胞因子は,ほとんど未定義のままである.

研究 の 目的:

  • 動物性パピローマウイルスがミトスの染色体に留まることに関与する細胞タンパク質機構を特定する.
  • ウイルスのエピソーム分割に関与する宿主因子に関する最初の洞察を提供すること.

主な方法:

  • この研究では,相互作用するパートナーを特定するために,タンパク質学的アプローチを使用した可能性が高い.
  • 免疫プレシピテーションや顕微鏡などの技術は,おそらく相互作用と局所化を検証するために使用されました.
  • ウイルスの保持における特定されたタンパク質の役割を評価するために,機能的測定が行われた可能性が高い.

主要な成果:

  • 研究では,動物性パピローマウイルスエピソードと相互作用する特定の細胞タンパク質を特定しました.
  • これらのタンパク質は,ウイルスのDNAをミトスの染色体と結びつけるのに不可欠です.
  • この発見は,ウイルスのゲノム安定性と伝播のための重要なメカニズムを明らかにしています.

結論:

  • この研究は,動物性パピローマウイルス染色体結合に関与する重要な宿主細胞因子を明らかにしています.
  • これは,ウイルスの持続メカニズムについての基本的な理解を提供します.
  • これらの発見は,ウイルスの保持を標的とした治療戦略の道を開く.