人間のエンドヌクレアース複合体の識別は,tRNAスプライシングとプレ-mRNA3'エンド形成の間のリンクを明らかにする
Sergey V Paushkin1, Meenal Patel, Bansri S Furia
1PTC Therapeutics, 100 Corporate Court, South Plainfield, NJ 07080, USA.
Cell
|April 28, 2004
まとめ
研究者はヒトのtRNAスプライシングエンドヌクレアスを特定し,そのサブユニットとプレ-mRNA処理へのリンクを明らかにしました. この発見は,細胞の成長に必要なRNA処理イベントに光を当てています.
科学分野:
- 分子生物学は分子生物学である.
- RNA 処理 RNA 処理
- 細胞生物学 細胞生物学
背景:
- tRNAスプライシングは,細胞の成長と分裂に不可欠であり,イントロンの除去が含まれています.
- イーストのtRNAスプライシングエンドヌクレアゼはよく特徴づけられているが,哺乳類の酵素は未確認のままである.
研究 の 目的:
- 人間のtRNAスプライシングエンドヌクレアースを特定し,特徴づけること.
- 特定されたサブユニットの機能と,他のRNA処理経路との関連を調査する.
主な方法:
- タンパク質複合体の識別と特徴付け.
- イーストエンドヌクレアースサブユニットによるホモロジー分析.
- siRNA媒介による遺伝子枯渇とRNA成熟の欠陥の分析.
主要な成果:
- HsSen2,HsSen34,HsSen15,およびHsSen54.4を含むヒトtRNAスプライシングエンドヌクレアース複合体の識別
- 異なったエンドヌクレアース複合体を形成するSEN2同型の代替スプライス発見.
- 2つのヒトエンドヌクレアゼ複合体とプレ-mRNA 3'エンド処理因子との関連.
- siRNAのSEN2の枯渇は,プレ-tRNAとプレ-mRNAの成熟の両方で欠陥を引き起こしました.
結論:
- ヒトのtRNAスプレイスングエンドヌクレアースサブユニットが識別され,酵母類の同類に同類性がある.
- プレ-tRNAスプライシングとプレ-mRNA3'エンド形成の間の新しいリンクが確立されています.
- エンドヌクレアスのサブユニットは,複数のRNA処理イベントに参加し,細胞の成長と分裂に影響を与える可能性があります.
関連する概念動画
01:32RNA Splicing
Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
02:01pre-mRNA Processing
In eukaryotic cells, transcripts made by RNA polymerase are modified and processed before exiting the nucleus. Unprocessed RNA is called precursor mRNA or pre-mRNA to distinguish it from mature mRNA.
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a “cap” to the 5’ end of the growing transcript. In this process, a 5’ phosphate is replaced by modified guanosine that has a methyl group attached to it (7-Methyl guanosine). This 5’ cap helps the...
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a “cap” to the 5’ end of the growing transcript. In this process, a 5’ phosphate is replaced by modified guanosine that has a methyl group attached to it (7-Methyl guanosine). This 5’ cap helps the...
02:01Pre-mRNA Processing
In eukaryotic cells, transcripts made by RNA polymerase are modified and processed before exiting the nucleus. Unprocessed RNA is called precursor mRNA or pre-mRNA to distinguish it from mature mRNA.
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a “cap” to the 5’ end of the growing transcript. In this process, a 5’ phosphate is replaced by modified guanosine that has a methyl group attached to it (7-Methyl guanosine). This 5’ cap helps the...
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a “cap” to the 5’ end of the growing transcript. In this process, a 5’ phosphate is replaced by modified guanosine that has a methyl group attached to it (7-Methyl guanosine). This 5’ cap helps the...
01:32RNA Splicing
Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
02:18Alternative RNA Splicing
Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
01:32Pre-mRNA Processing: RNA Splicing
Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...