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Non-invasive Assessment of Microvascular and Endothelial Function
Published on: January 29, 2013
内皮機能における人種特有の差異:アフリカ系アメリカ人の血管疾患への傾向
Leszek Kalinowski1, Iwona T Dobrucki, Tadeusz Malinski
1Department of Biochemistry, Ohio University, Athens, OH 45701, USA.
Circulation
|May 26, 2004
まとめ
黒人からの内皮細胞は,酸化窒素 (NO) が減少し,超酸化窒素 (O2-) と過酸化窒素 (ONOO-) が増加し,白人と比較して血管機能障害と心血管疾患のリスクに貢献しています.
科学分野:
- 血管生物学 血管生物学
- バイオケミストリー バイオケミストリー
- 細胞生理学 細胞生理学
背景:
- 高血圧と糖尿病の合併症は,白人の集団よりも黒人の集団でより深刻です.
- 超酸化物 (O2-) と酸化窒素 (NO) の相互作用により,過酸化ニートリート (ONOO-) が形成され,血管の病理生理学において重要な役割を果たします.
- 内皮 NO/O2-/ONOO-代謝における人種差異は,黒人個人を内皮機能不全に誘発する可能性がある.
研究 の 目的:
- 内皮の酸化窒素 (NO),超酸化窒素 (O2-),過酸化ニートリート (ONOO-) の代謝における人種差異を調査する.
- 黒人個人の内皮機能不全に貢献する根本的なメカニズムを決定する.
主な方法:
- 高感度電気化学ナノセンサを使用して,黒と白のドナーからヒト静脈内皮細胞 (HUVEC) のNO/O2-/ONOO-放出量を測定しました.
- 分析されたNAD(P) H-酸化酵素と内皮 NO合成酵素 (eNOS) の活性と発現は,西側の免疫ボルトリングと酵素アッセイによるものです.
主要な成果:
- 黒人個体からのHUVECは,白人の個体と比較して,生物活性なNOの放出が減少し,O2とONOOの放出が増加した.
- 黒人個人のHUVECにおけるeNOS発現の増加は,O2-.によってNOの分解が強化されたため,NOの生物利用性が低下したとの関連があった.
- 主にNAD(P) H酸化酵素と結合されていないeNOSから発生する高濃度のO2生成は,酸化ストレスの増加に寄与する.
結論:
- 黒人の内皮細胞のNO/O2-/ONOO-バランスは,内皮機能不全に関連した酸化還元状態にシフトします.
- これらの代謝の変化は,内皮機能不全と心血管合併症への人種的予備性を説明する可能性がある.
- 酸化ストレスによる強化されたNO不活性化は,黒人個人の内皮機能不全の特徴です.
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