タンパク質フォスファタゼ1の構造的基礎 調節
Mohammed Terrak1, Frederic Kerff, Knut Langsetmo
1Boston Biomedical Research Institute, 64 Grove Street, Watertown, Massachusetts 02472, USA.
Nature
|May 28, 2004
まとめ
プロテイン・フォスファタゼ1 (PP1) は,MYPT1.1.のような規制サブユニットを通じて基板特異性を獲得します. 構造分析は,MYPT1がPP1をどのように形づくっているかを明らかにしています.
科学分野:
- バイオケミストリー バイオケミストリー
- 分子生物学は分子生物学である.
- 構造生物学 構造生物学とは
背景:
- セリン/スレオニン (Ser/Thr) タンパク質キナーゼとフォスファタゼは,リン酸化による生物学的プロセスを調節する.
- プロテイン・フォスファタゼ1 (PP1) は細胞機能に不可欠であるが,固有の基板特異性がない.
- 通常,RVxFモチーフを含む規制サブユニットは,PP1に特異性を与える.
研究 の 目的:
- PP1-MYPT1複合体の結晶構造を決定するために.
- MYPT1がPP1の活動と特異性をどのように調節するかを理解する.
- PP1基板ターゲティングの構造的基礎を解明する.
主な方法:
- 2.7 Å の解像度のX線結晶学.
- MYPT1.1.のN端ドメインとタンパク質フォスファタゼ1 (PP1) の共結晶化.
- PP1-MYPT1複合体の構造分析.
主要な成果:
- PP1-MYPT1複合体の結晶構造が決定されました.
- MYPT1結合により,PP1の触媒裂け目が大きく変化する.
- MYPT1のRVxFモチーフの側面にある構造要素は,この再構成とミオシン特異性の鍵です.
結論:
- MYPT1結合は,形状の変化によってPP1にミオシン特異性を与える.
- 明らかにされた構造は,さまざまなサブユニットによるPP1規制の洞察を提供します.
- このメカニズムは,他のPP1の調節相互作用を理解するために一般化できます.
関連する概念動画
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02:54Protein Kinases and Phosphatases
Proteins undergo chemical modifications that trigger changes in the charge, structure, and conformation of the proteins. Phosphorylation, acetylation, glycosylation, nitrosylation, ubiquitination, lipidation, methylation, and proteolysis are various protein modifications that regulate protein activity. Such modifications are usually enzyme-driven.
Protein kinases
Many proteins in the cell are regulated by phosphorylation, the addition of a phosphate group. A family of enzymes called kinases...
Protein kinases
Many proteins in the cell are regulated by phosphorylation, the addition of a phosphate group. A family of enzymes called kinases...
02:04Covalently Linked Protein Regulators
Proteins can undergo many types of post-translational modifications, often in response to changes in their environment. These modifications play an important role in the function and stability of these proteins. Covalently linked molecules include functional groups, such as methyl, acetyl, and phosphate groups, and also small proteins, such as ubiquitin. There are around 200 different types of covalent regulators that have been identified.
These groups modify specific amino acids in a protein.
These groups modify specific amino acids in a protein.
02:58Regulated Protein Degradation
It is vital to regulate the activity of enzymatic as well as non-enzymatic proteins inside the cell. This can be achieved either through creating a balance between their rate of synthesis and degradation or regulating the intrinsic activity of the protein. Both these regulation mechanisms play an essential role in the normal functioning of cells.
Protein degradation plays two important roles in the cells. It helps to protect cells from misfolded or damaged proteins before they lead to a...
Protein degradation plays two important roles in the cells. It helps to protect cells from misfolded or damaged proteins before they lead to a...