Jove
Visualize
お問い合わせ
JoVE
x logofacebook logolinkedin logoyoutube logo
JoVEについて
概要リーダーシップブログJoVEヘルプセンター
著者向け
出版プロセス編集委員会範囲と方針査読よくある質問投稿
図書館員向け
推薦の声購読アクセスリソース図書館諮問委員会よくある質問
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experimentsアーカイブ
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教員リソースセンター教員サイト
利用規約
プライバシーポリシー
ポリシー

関連する概念動画

What is Gene Expression?01:42

What is Gene Expression?

Overview
Gene expression is the process in which DNA directs the synthesis of functional products, that is, proteins. Cells can regulate gene expression at various stages. It allows organisms to generate different cell types and enables cells to adapt to internal and external factors.
Genetic Information Flows from DNA to RNA to Protein
A gene is a stretch of DNA that serves as the blueprint for functional RNAs and proteins. Since DNA is made up of nucleotides and proteins consist of amino...
Regulation of Expression Occurs at Multiple Steps02:24

Regulation of Expression Occurs at Multiple Steps

Gene expression can be regulated at almost every step from gene to protein. Transcription is the step that is most commonly regulated. This involves the binding of proteins to short regulatory sequences on the DNA. This association can either promote or inhibit the transcription of a gene associated with the respective sequence.
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
mRNA Stability and Gene Expression02:51

mRNA Stability and Gene Expression

The structure and stability of mRNA molecules regulates gene expression, as mRNAs are a key step in the pathway from gene to protein. In eukaryotes, the half-life of mRNA varies from a few minutes up to several days. mRNA stability is essential in growth and development. The absence of the proteins regulating its stability, such as tristetraprolin in mice, can cause systemic issues, including bone marrow overgrowth, inflammation, and autoimmunity.
Cis-acting Elements involved in mRNA stability
What is Gene Expression?01:36

What is Gene Expression?

A gene is a stretch of DNA that serves as the blueprint for functional RNAs and proteins. Since DNA is comprised  of nucleotides and proteins are comprised of amino acids, a mediator is required to convert the information encoded in DNA into proteins. This mediator is the messenger RNA (mRNA). mRNA copies the blueprint from DNA by a process called transcription. In eukaryotes, transcription occurs in the nucleus by complementary base-pairing with the DNA template. The mRNA is then processed and...
Regulation of Expression Occurs at Multiple Steps02:24

Regulation of Expression Occurs at Multiple Steps

Gene expression can be regulated at almost every step from gene to protein. Transcription is the step that is most commonly regulated. This involves the binding of proteins to short regulatory sequences on the DNA. This association can either promote or inhibit the transcription of a gene associated with the respective sequence.
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
Regulation of Expression at Multiple Steps01:23

Regulation of Expression at Multiple Steps

The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the addition of a...

こちらも読む

関連記事

共著者、ジャーナル、引用グラフによってこの研究に関連する記事。

並び替え
Same author

Single-Particle Tracking of AMPA Receptor-Containing Vesicles.

Bio-protocol·2025
Same author

Activity-dependent synapse elimination requires caspase-3 activation.

eLife·2025
Same author

Plasticity-induced actin polymerization in the dendritic shaft regulates intracellular AMPA receptor trafficking.

eLife·2024
Same author

Correction: Evolution of reduced co-activator dependence led to target expansion of a starvation response pathway.

eLife·2022
Same author

The LRRK2 G2019S mutation alters astrocyte-to-neuron communication via extracellular vesicles and induces neuron atrophy in a human iPSC-derived model of Parkinson's disease.

eLife·2021
Same author

Increasing gender diversity in the STEM research workforce.

Science (New York, N.Y.)·2019

関連する実験動画

Updated: Jul 13, 2026

Processing of Primary Brain Tumor Tissue for Stem Cell Assays and Flow Sorting
08:14

Processing of Primary Brain Tumor Tissue for Stem Cell Assays and Flow Sorting

Published on: September 25, 2012

ユカリオットの遺伝子発現におけるストキャスティシティの制御

Jonathan M Raser1, Erin K O'Shea

  • 1Department of Biochemistry and Biophysics, Howard Hughes Medical Institute, University of California-San Francisco (UCSF), 600 16th Street, Room S472D, San Francisco, CA 94143-2240, USA.

Science (New York, N.Y.)
|May 29, 2004
PubMed
まとめ

遺伝子発現のノイズ,またはランダムな変動は,細胞の多様性を引き起こします. この騒音は遺伝子特異であり,進化し,細胞の忠誠性と多様性をバランスさせることができる.

科学分野:

  • 分子生物学は分子生物学である.
  • 遺伝学 遺伝学とは
  • システム生物学 システム生物学

背景:

  • 細胞の行動は,遺伝子発現のランダムな変動 (ノイズ) によって変化することがあります.
  • 遺伝子発現騒音の発生源と影響を理解することは,分子生物学において極めて重要です.

研究 の 目的:

  • ユーカリオットの遺伝子発現における内在的なノイズを定量化するために.
  • 遺伝子発現の変動に影響を与える要因を調査する.
  • 遺伝子発現騒音の進化的可能性を調査する.

主な方法:

  • 二倍体細胞における2つのアレルの発現の違いを定量化して,固有のノイズを測定する.
  • プロモーターの活性化と転写をメッセンジャーRNA (mRNA) の変動と結びつけるモデルを開発した.
  • 遺伝子発現ノイズに影響を与えるシスおよびトランス作用の突然変異を特定しました.

主要な成果:

  • 遺伝子発現ノイズは遺伝子特異であり,規制経路や発現率とは無関係です.
  • プロモーターの活性化と転写バランスがmRNAの変動性に影響するモデルが提案されました.
  • 騒音を変化させる変異が特定され,騒音が進化可能な特徴であることを支持しました.

さらに関連する動画

Sealable Femtoliter Chamber Arrays for Cell-free Biology
13:44

Sealable Femtoliter Chamber Arrays for Cell-free Biology

Published on: March 11, 2015

Single-cell Gene Expression Profiling Using FACS and qPCR with Internal Standards
10:50

Single-cell Gene Expression Profiling Using FACS and qPCR with Internal Standards

Published on: February 25, 2017

関連する実験動画

Last Updated: Jul 13, 2026

Processing of Primary Brain Tumor Tissue for Stem Cell Assays and Flow Sorting
08:14

Processing of Primary Brain Tumor Tissue for Stem Cell Assays and Flow Sorting

Published on: September 25, 2012

Sealable Femtoliter Chamber Arrays for Cell-free Biology
13:44

Sealable Femtoliter Chamber Arrays for Cell-free Biology

Published on: March 11, 2015

Single-cell Gene Expression Profiling Using FACS and qPCR with Internal Standards
10:50

Single-cell Gene Expression Profiling Using FACS and qPCR with Internal Standards

Published on: February 25, 2017

結論:

  • 本質的な遺伝子発現ノイズは,遺伝子特異的な特徴である.
  • プロモーターの活性化と転写のバランスは,mRNAレベル変動の重要な要因です.
  • 遺伝子発現騒音は,細胞の適応に最適化され,一貫性と多様性をバランスさせることができる進化可能な特徴です.