電圧ゲートCa2+チャネルのα1-βサブユニット相互作用の構造的基礎
Yu-Hang Chen1, Ming-Hui Li, Yun Zhang
1Department of Biological Sciences, Columbia University, New York, New York 10027, USA.
Nature
|June 1, 2004
まとめ
ベータサブユニットです.
科学分野:
- 分子生物学は分子生物学である.
- 神経科学は神経科学である.
- 構造生物学 構造生物学とは
背景:
- 高電圧で活性化されたCa2+チャネルは,生物学的プロセスにとって極めて重要です.
- ベータサブユニットは,Ca2+チャネルの表面表現と機能に不可欠です.
- アルファ1-ベータサブユニットの相互作用の正確な分子メカニズムは不明である.
研究 の 目的:
- Ca2+チャネルにおけるα1-βサブユニット相互作用の構造的基礎を解明する.
- この相互作用におけるベータ相互作用領域 (BID) の役割を決定する.
主な方法:
- X線結晶学を用いてβ3およびβ4サブユニットの構造を決定した.
- ベータ3単独,ベータ4単独,およびアルファ1相互作用領域 (AID) と複合したベータ3の構造が得られた.
主要な成果:
- ベータサブユニットのコアは,SH3とガニラートキナーゼ (GK) の2つのドメインで構成されています.
- AIDは,GKドメインの水害性溝に結合し,高親和相互作用を確立します.
- BIDは構造的整合性にとって不可欠ですが,alpha1.1.を直接結合することはありません.
結論:
- ベータサブユニットのGKドメインは,アルファ1サブユニットのAID.に高親和結合を直接媒介する.
- SH3とGKドメインを持つベータサブユニットのモジュール構造は,多様なタンパク質相互作用能力を示唆しています.
- この構造的な洞察は,Ca2+チャネル調節における多機能タンパク質としてのβサブユニットの役割について新しい視点を提供します.
さらに関連する動画
13:40Live Cell Calcium Imaging Combined with siRNA Mediated Gene Silencing Identifies Ca2+ Leak Channels in the ER Membrane and their Regulatory Mechanisms
Published on: July 7, 2011
08:25Voltage and Calcium Dual Channel Optical Mapping of Cultured HL-1 Atrial Myocyte Monolayer
Published on: March 23, 2015
関連する概念動画
01:19The Role of Ion Channels in Neuronal Computation
A postsynaptic neuron usually receives numerous impulses from several other presynaptic neurons. The axon hillock of the postsynaptic neuron integrates all these signals and determines the likelihood of firing an action potential.
Sometimes a single EPSP is strong enough to induce an action potential in the postsynaptic neuron. However, multiple presynaptic inputs must often create EPSPs around the same time for the postsynaptic neuron to be sufficiently depolarized to fire an action potential.
Sometimes a single EPSP is strong enough to induce an action potential in the postsynaptic neuron. However, multiple presynaptic inputs must often create EPSPs around the same time for the postsynaptic neuron to be sufficiently depolarized to fire an action potential.
01:26Voltage-gated Ion Channels
Voltage-gated ion channels are transmembrane proteins that open and close in response to changes in the membrane potential. They are present on the membranes of all electrically excitable cells such as neurons, heart, and muscle cells.
Generally, all voltage-gated ion channels have a 'voltage-sensing domain' that spans the lipid bilayer. The charged residues in the sensor move in response to the membrane potential changes that open the channel allowing ions movement. There are several types of...
Generally, all voltage-gated ion channels have a 'voltage-sensing domain' that spans the lipid bilayer. The charged residues in the sensor move in response to the membrane potential changes that open the channel allowing ions movement. There are several types of...
01:27Feedback Regulation of Calcium Concentration
Calcium is an essential signaling molecule required for various cellular functions. Calcium pumps and ion channels on cell and organellar membranes, such as those on the endoplasmic reticulum (ER), regulate calcium concentrations inside the cell. They remain closed, keeping the cytosolic calcium levels low at a resting state.
Various transmembrane receptors, such as G protein-coupled receptors (GPCRs), elicit a response to extracellular signals by increasing cytosolic calcium. Activated GPCRs...
Various transmembrane receptors, such as G protein-coupled receptors (GPCRs), elicit a response to extracellular signals by increasing cytosolic calcium. Activated GPCRs...
01:16Calmodulin-dependent Signaling
Calmodulin (CaM) is a calcium-binding protein in eukaryotes that controls various calcium-regulated cellular processes. It has four calcium-binding sites that bind calcium to form the calcium-calmodulin ( Ca2+-CaM) complex. GPCR stimulation increases the calcium levels in the cells that bind to CaM and induces a conformational change.
The Ca2+-CaM complex does not have enzymatic activity by itself. Instead, the complex binds downstream target proteins, including membrane proteins or enzymes,...
The Ca2+-CaM complex does not have enzymatic activity by itself. Instead, the complex binds downstream target proteins, including membrane proteins or enzymes,...
01:30Ligand-Gated Ion Channel Receptor: Gating Mechanism
Ligand-gated ion channels are transmembrane proteins that play a vital role in intercellular communication and functions of the nervous system. They allow the influx of ions across the membrane once the neurotransmitter binds, allowing the subsequent transmission of electrical excitation across the neurons. Other ligand-gated ion channels, like the γ-aminobutyric acid (GABA) receptor, permit anions like chloride into the cells on the binding of the GABA molecule. Their entry into the cell...
01:26Voltage-gated Ion Channels
Voltage-gated ion channels are transmembrane proteins that open and close in response to changes in the membrane potential. They are present on the membranes of all electrically excitable cells such as neurons, heart, and muscle cells.
Generally, all voltage-gated ion channels have a 'voltage-sensing domain' that spans the lipid bilayer. The charged residues in the sensor move in response to the membrane potential changes that open the channel allowing ions movement. There are several types of...
Generally, all voltage-gated ion channels have a 'voltage-sensing domain' that spans the lipid bilayer. The charged residues in the sensor move in response to the membrane potential changes that open the channel allowing ions movement. There are several types of...