MSX1はヒストンH1bと協働し,転写とミオゲネシスを阻害する
Hansol Lee1, Raymond Habas, Cory Abate-Shen
1Center for Advanced Biotechnology and Medicine, University of Medicine and Dentistry of New Jersey (UMDNJ)-Robert Wood Johnson Medical School, Piscataway, NJ 08854, USA.
Science (New York, N.Y.)
|June 12, 2004
まとめ
Msx1とヒストンH1bの相互作用により,骨格筋の遺伝子発現を抑制することがわかった. この発見は,開発中の特定の遺伝子転写を制御するリンクラーヒストンの新たな役割を明らかにしています.
科学分野:
- 発達生物学 発達生物学とは
- 分子生物学は分子生物学である.
- エピジェネティクス エピジェネティクス
背景:
- 骨格筋の分化が胚の発達に不可欠である.
- Msxホメオプロテインを含む転写因子は,このプロセスを調節する.
- ミオゲン遺伝子の抑制におけるMsx1の特定の役割については,さらなる解明が必要である.
研究 の 目的:
- ミオゲン遺伝子発現を抑制するMsx1の機能を調査する.
- 筋肉の分化に関与する Msx1 の新しいタンパク質相互作用を特定する.
- Msx1が骨格筋の発達を調節するメカニズムを理解するために.
主な方法:
- 菌根性遺伝子発現におけるMsx1の機能を調査した.
- Msx1とヒストンH1b.との間の物理的な相互作用が特定されました.
- Msx1とH1bがMyoDの規制要素に結合することを分析した.
- 細胞培養とXenopus動物キャップにおける筋肉の分化抑制の評価.
主要な成果:
- Msx1はマウスヒストンのH1同型であるヒストンH1bと物理的に相互作用する.
- Msx1とH1bは,MyoD.の重要な規制要素に結合する.
- この結合は,抑制されたクロマチンの形成を誘導する.
- Msx1とH1bは, in vitroとin vivoで筋肉の分化を抑制するために協力します.
結論:
- 遺伝子特異的な転写調節におけるリンカーヒストンの新しい機能を定義した.
- Msx1とH1bは,骨格筋の分化を抑制する協力的な役割を果たします.
- この相互作用は,筋肉発達の表遺伝子制御に関する新しい洞察をもたらします.
関連する概念動画
01:33Pleiotropy
Pleiotropy is the phenomenon in which a single gene impacts multiple, seemingly unrelated phenotypic traits. For example, defects in the SOX10 gene cause Waardenburg Syndrome Type 4, or WS4, which can cause defects in pigmentation, hearing impairments, and an absence of intestinal contractions necessary for elimination. This diversity of phenotypes results from the expression pattern of SOX10 in early embryonic and fetal development. SOX10 is found in neural crest cells that form melanocytes,...
02:16Transcription Factors
Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
02:25Spreading of Chromatin Modifications
The histone proteins in the nucleosomes are post-translationally modified (PTM) to increase or decrease access to DNA. The commonly observed PTMs are methylation, acetylation, phosphorylation, and ubiquitination of lysine amino acids in the histone H3 tail region. These histone modifications have specific meaning for the cell. Hence, they are called "histone code". The protein complex involved in histone modification is termed as "reader-writer" complex.
Writers
The writer is an enzyme that can...
Writers
The writer is an enzyme that can...
02:23Master Transcription Regulators
Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
01:13Formation of Muscle Fibers from Myoblasts
De novo myogenesis, or the formation of muscle fibers, begins during the early embryonic stages. The skeletal muscle is formed from somites– blocks of embryonic cell layers. The somites are further divided into dermatomes, myotomes, sclerotomes, and syndetomes. Among these, the myotomes give rise to muscle fibers.
Muscle progenitor cells (MPCs) are formed from the myotomes. MPCs express genes that encode the transcription factors Pax3 and Pax7. Along with Pax 3/7, other transcription factors...
Muscle progenitor cells (MPCs) are formed from the myotomes. MPCs express genes that encode the transcription factors Pax3 and Pax7. Along with Pax 3/7, other transcription factors...
01:30General Transcription Factors
Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...