自己組み立てペプチドは,水害性化合物の潜在的キャリアとして機能する
Christine Keyes1, Jean Duhamel, Shan-Yu Fung
1Department of Chemistry , University of Waterloo, 200 University Avenue West, Waterloo, Ontario N2L 3G1, Canada.
Journal of the American Chemical Society
|June 17, 2004
まとめ
セルフアセンブリオリゴペプチドEAK16 IIは,水中での水害性の負荷を安定させ,それをリポフィル環境に運びます. 放出速度は,ペプチドと貨物の比率とコーティングの厚さを調整することによって調整できます.
科学分野:
- バイオマテリアル科学 バイオマテリアル科学
- 超分子化学 超分子化学
- 薬物の配送システムです.
背景:
- 水性化合物は,水性 суспенションの安定化を必要とします.
- 自己組み立てペプチドは,貨物の安定化のための有望なアプローチを提供します.
- 生物膜を模倣することは,貨物輸送を理解するために非常に重要です.
研究 の 目的:
- EAK16 II.を使用した水害性の貨物の安定化と放出を調査する.
- EAK16 IIコーティングから脂質ベジクルへのピレンの放出運動を定量化するために.
- 解放率に対するペプチド対貨物比とコーティング厚さの影響を決定する.
主な方法:
- EAK16 II オリゴペプチドによるピレンマイクロ結晶の安定化.
- 卵のフォスファディチルコリン (EPC) 膀を調製して細胞膜をモデル化します.
- ピレン放出運動をモニターするための光スペクトロスコーピー.
- スキャン電子顕微鏡 (SEM) は,結晶のコーティング厚さを分析します.
主要な成果:
- EAK16 IIは水性溶液中の水性ピレン結晶を効果的に安定させました.
- ピレンはEAK16 IIのコーティングから放出され,EPCベシクル内に分子的に分散した.
- 放出率はペプチドとピレンの比率とコーティングの厚さに左右され,比率が高く,コーティングが厚くなると放出が遅くなる.
結論:
- EAK16 IIは,水中環境における水害性貨物の安定化のための実用的なシステムです.
- EAK16 IIシステムは,脂質二層のようなリポフィル環境への貨物の輸送を容易にする.
- 調節可能な放出運動は,ペプチド対貨物比とコーティング形態学の制御によって達成できます.
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