関連する実験動画

Updated: Jul 10, 2026

Thermodynamics of Membrane Protein Folding Measured by Fluorescence Spectroscopy
10:09

Thermodynamics of Membrane Protein Folding Measured by Fluorescence Spectroscopy

Published on: April 28, 2011

折り畳み可能な景観の微細構造の探査として検出依存の運動学

Wei Yuan Yang1, Martin Gruebele

  • 1Center for Biophysics and Computational Biology and Department of Chemistry, University of Illinois at Urbana-Champaign, Illinois 61801, USA. weiyang@fas.harvard.edu

Journal of the American Chemical Society
|June 24, 2004
PubMed
まとめ

タンパク質の折り畳み風景には,遅いプロリンイソメリゼーションから速いサブマイクロ秒マイクロ構造まで,多くのエネルギー最小値があります. 波長に依存する運動学は,これらの隠されたタンパク質の折り畳み動力学とエネルギー景観の荒さを示しています.

さらに関連する動画

Microfluidic Mixers for Studying Protein Folding12:42

Microfluidic Mixers for Studying Protein Folding

Published on: April 10, 2012

Measuring Biomolecular DSC Profiles with Thermolabile Ligands to Rapidly Characterize Folding and Binding Interactions09:15

Measuring Biomolecular DSC Profiles with Thermolabile Ligands to Rapidly Characterize Folding and Binding Interactions

Published on: November 21, 2017

関連する概念動画

Protein Folding01:22

Protein Folding

Overview
Protein Folding01:22

Protein Folding

Overview
Molecular Chaperones and Protein Folding03:00

Molecular Chaperones and Protein Folding

The native conformation of a protein is formed by interactions between the side chains of its constituent amino acids. When the amino acids cannot form these interactions, the protein cannot fold by itself and needs chaperones. Notably, chaperones do not relay any additional information required for the folding of polypeptides; the native conformation of a protein is determined solely by its amino acid sequence. Chaperones catalyze protein folding without being a part of the folded protein.
The...
Protein Folding01:25

Protein Folding

Proteins are chains of amino acids linked together by peptide bonds. Upon synthesis, a protein folds into a three-dimensional conformation, critical to its biological function. Interactions between its constituent amino acids guide protein folding, and hence the protein structure is primarily dependent on its amino acid sequence.
Protein Structure Is Critical to Its Biological Function
Proteins perform a wide range of biological functions such as catalyzing chemical reactions, providing...
Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...