人間の脂肪組織由来幹細胞による産後新血管化の改善
A Miranville1, C Heeschen, C Sengenès
1Department of Cardiovascular Physiology, J-W Goethe University, Frankfurt, Germany.
Circulation
|July 9, 2004
まとめ
脂肪組織のストロマ血管分子は,内皮原細胞として機能するCD34+/CD31-細胞を含んでいます. これらの細胞は,血管形成を改善し,細胞治療に役立つかもしれません.
科学分野:
- 再生医学は,再生医療である.
- 細胞生物学 細胞生物学
- 血管生物学 血管生物学
背景:
- 脂肪組織 (AT) のストロマ血管分 (SVF) は,幹細胞を含むことが知られている.
- これらの幹細胞の特徴づけは,治療応用において極めて重要です.
研究 の 目的:
- ヒトの脂肪組織SVF.内の幹細胞と祖先細胞を特定し,特徴づけること.
- 血管新生を促進するこれらの細胞の治療の可能性を評価する.
主な方法:
- CD31,CD34,CD133,ABCG2.0に対する抗体を用いて,光活性化細胞分類 (FACS) 分析を行った.
- CD34+/CD31-細胞を磁石マイクロビーズで隔離する.
- マウスの不血性後肢モデルにおけるin vitro差別化アッセイおよびin vivo研究.
主要な成果:
- 人間のAT SVFには,CD133とABCG2.2を発現する集団を含むCD34+細胞が含まれています.
- CD31発現に基づいて2つの異なるCD34+細胞集団が特定されました.
- CD34+/CD31-細胞がインビトロで内皮細胞に微分化した.
- CD34+/CD31-細胞の静脈内注射は,不血性後肢における血流と毛細血管の密度を改善しました.
結論:
- 人間のATSVFにおける独特のCD34+/CD31-細胞群は,内皮原始細胞の特徴を示している.
- 人間の脂肪組織は,治療的血管生成のための幹細胞/祖先細胞の潜在的な源である.
- これらの発見は,再生療法のための脂肪系由来幹細胞の使用を支持しています.
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