心臓の記憶は,老化とともに,一時的な外向きの電流の発達と関連して進化する
Alexei N Plotnikov1, Eugene A Sosunov, Kornelis W Patberg
1Center for Molecular Therapeutics, Department of Pharmacology, College of Physicians and Surgeons of Columbia University, New York, NY 10032, USA.
Circulation
|July 21, 2004
まとめ
心臓記憶 (CM) は,カルシウム無感の一時的な外向き電流 (I(to)) の成熟により,年齢とともに発達する. このプロセスは,心臓の行動ポテンシャル・ノッチと関連する遺伝子発現の変化を伴い,心臓の電気再構成能力に影響を与えます.
科学分野:
- 心臓病学 心臓病学
- 電気生理学 電気生理学
- 発達生物学 発達生物学とは
背景:
- カルシウム無感の臨時向外電流 (I(to)) は,心臓の記憶 (CM) に重要な役割を果たしています.
- 心臓の記憶は,心臓の電気生理学的改造能力を反映しています.
- この研究は,CM進化の原動力としてのI ((to)) の発達成熟を調査しています.
研究 の 目的:
- 心臓記憶の進化は,Iの発達的成熟によって引き起こされるという仮説を検証する.
- 心臓の電気生理学的な改造における年齢に依存する変化を理解するために.
主な方法:
- 心臓ペースは,異なる年齢の犬 (1日から成人まで) でCMを誘導するために使用されました.
- CM.を定量化するために,T波ベクトル位移 (TVD) を測定した.
- マイクロエレクトロドの研究では,心上運動ポテンシャルのノッチ特徴を評価した.
- KChIP2およびKv4.3のmRNA発現はRT-PCRを用いて定量化されました.
主要な成果:
- TVDは,新生児よりも成人の方が著しく多く,年齢とともに増加し,大体200日後に成人のレベルに達しました.
- 新生児には存在しない心臓のアクションポテンシャル・ノッチは,年齢とともに明らかになり,深まりました.
- ノッチマグニチュードとTVDの間に正の相関が観察されました.
- KChIP2とKv4.3のmRNAレベルは年齢とともに増加し,CMの発達を反映した.
結論:
- 心臓記憶の誘導性は,年齢とともに増加します.
- この年齢に依存する増加は,心臓のアクションポテンシャル・ノッチとKChIP2/Kv4.3 mRNA発現の進化と関連しています.
- 発達中の電気生理学的改造と記憶に対する心臓の能力は,部分的にI (((to)) の決定因子の成熟に依存しています.
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