Escherichia coliの細胞分裂遺伝子ftsZは,新しいGTP結合タンパク質をコードする

D RayChaudhuri¹, J T Park

¹Department of Molecular Biology and Microbiology, Tufts University School of Medicine, Boston, Massachusetts 02111.

Nature

|September 17, 1992

まとめ

エシェリキア大腸菌の細胞分裂には,GTPと結合し,GTPアゼ活性を持つFtsZタンパク質が含まれています. これは,FtsZが細胞分離を開始する分子スイッチとして作用することを示唆しています.

科学分野:

微生物学微生物学とは
分子生物学は分子生物学である.
細胞生物学細胞生物学

背景:

Escherichia coliの細胞分裂はセプテーションに依存し,その生化学的メカニズムはほとんど不明のままである.
遺伝学的研究は,E. coli分離の初期段階におけるftsZ (フィラメント温度感受性Z) 遺伝子の中心的な役割を強調しています.

研究の目的:

Escherichia coliの細胞分裂におけるFtsZタンパク質の生化学的機能を調査する.
FtsZがGTP結合とGTP酵素の活性を示しているかどうかを決定します.

主な方法:

精製されたFtsZタンパク質を用いたGTP結合試験をインビトロで行いました.
GTPase活性アッセイは,Mg2+) 依存性を有する.
条件付き-致死性アレル (ftsZ84) のFtsZタンパク質の分析.
結合核酸と膜結合を特定するための共同浄化研究.

主要な成果:

精製されたFtsZタンパク質は,ユニークな配列要素を通じてGTP結合を示しています.
FtsZはMg(2+) 依存のGTPase活性を示すが,FtsZ84変異タンパク質では著しく減少している.
FtsZはGDPと共浄化し,in vivoのGTPaseサイクルを示している.
FtsZの一部は膜関連であり,GTPと結合する.

結論:

FtsZはGTP結合タンパク質としてGTP酵素活性を持ち,細胞分裂中のGTP酵素サイクルに参加する可能性が高い.
FtsZ84のGTP結合とGTP酵素活性が低下していることから,分離における重要な役割が示唆される.
膜結合とGTP結合特性により,FtsZは,E. coli分離の開始を調節するGTP活性化された信号伝導経路に含まれる.

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