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Breast Milk Enhances Growth of Enteroids: An Ex Vivo Model of Cell Proliferation
Published on: February 15, 2018
合成哺乳類のプリオン
Giuseppe Legname1, Ilia V Baskakov, Hoang-Oanh B Nguyen
1Institute for Neurodegenerative Diseases, University of California, San Francisco, CA 94143, USA.
まとめ
研究者は,リコンビナントマウスプリオンタンパク質 (recMoPrP) のアミロイド繊維から感染性のプリオン菌株を作成しました. これらの線維がマウスの病気を引き起こし,プリオンが実際に感染性のタンパク質であることを示した.
科学分野:
- 神経科学は神経科学である.
- バイオケミストリー バイオケミストリー
- 感染症 感染症は感染症です.
背景:
- プリオンは,神経変性疾患に関与する誤った折りたたまれたタンパク質です.
- プリオンの伝染性性は確立されていますが,そのインビトロ生成は依然として困難です.
- 再結合プリオンタンパク質は,プリオン形成と感染性を研究するためのツールを提供します.
研究 の 目的:
- ポリメリ化再結合マウスプリオンタンパク質 (recMoPrP) が感染性プリオンを形成する可能性を調査する.
- recMoPrP.から生成されたプリオンの神経病理学的および伝播的特性を特徴づける.
主な方法:
- 再結合マウスプリオンタンパク質 (recMoPrP) は,アミロイド繊維にポリマー化されました.
- これらの線維は,マウスプリオンタンパク質 (MoPrP) を発現するトランス遺伝子マウスに脳内注射された.
- 疾患発症,タンパク質耐性PrPのウェスタン・ブロッティング,および野生型およびトランスジェニックマウスでの伝播研究が行われました.
主要な成果:
- 予防接種を受けたマウスは神経機能障害を発症し,脳抽出物でプロテアゼ耐性PrPを示した.
- 脳抽出物は,感染したマウスに病気を伝達し,PrP過剰発現したマウスのインキュベーション期間が短縮された.
- 神経病理学的分析は,新しいプリオン菌株の発生を示した.
結論:
- ポリマー化されたrecMoPrPは感染性のプリオンを形成することができる.
- これらの発見は,プリオンが感染性タンパク質であるという強力な証拠を提供します.
- この研究は,さらなる研究のために,感染性のプリオンをインビトロで生成する方法を提案しています.
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In SCNT, an egg cell is taken from an animal and its nucleus is removed, creating an enucleated egg. Then a somatic cell—any cell that is not a sex...
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