アデノウイルス初期1Bタンパク質による変換に必要なp53トランザクティベーションの阻害
1Department of Microbiology and Molecular Genetics, University of California, Los Angeles 90024-1570.
Nature
|May 7, 1992
まとめ
転写活性化剤である腫瘍抑制タンパク質p53は,細胞サイクル進行を阻害する. アデノウイルスタンパク質は,p53を阻害する.
科学分野:
- 分子生物学は分子生物学である.
- 腫瘍学 腫瘍学
- ウイルス学 ウイルス学 ウイルス学
背景:
- 細胞のフォスフォタンパク質p53は,細胞サイクル進行を阻害することによって腫瘍抑制剤として作用します.
- 細胞サイクル制御における転写活性化剤としてのp53の役割は完全に理解されていません.
- アデノウイルス2 (Ad2) E1B 55Kタンパク質は,変異細胞のp53と相互作用し,腫瘍形成に寄与する.
研究 の 目的:
- 細胞サイクル制御におけるp53の転写活性化の役割を調査する.
- アデノウイルスE1Bタンパク質によるp53の活性抑制と,その腫瘍性変異の可能性との相関を判定する.
主な方法:
- アデノウイルスE1B 55K変異体と野生型Ad12 E1B 54Kタンパク質の分析.
- これらのウイルスタンパク質がp53媒介の転写活性化を阻害する能力の評価.
- ウイルスのタンパク質とアデノウイルスE1Aとの間の協力の評価.
主要な成果:
- E1B変異体/変異体によるp53媒介の転写活性化の抑制と,細胞変異においてE1Aと協力する能力との間に強い相関が観察されました.
- E1B 55Kの変異体と野生型のAd12 E1B 54Kタンパク質は,この相関性を示した.
結論:
- p53は,転写因子として機能することで,細胞循環を抑制する可能性がある.
- アデノウイルスE1Bタンパク質がp53の転写活動を阻害する能力は,ウイルス腫瘍生成に極めて重要です.
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