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Mitogens and the Cell Cycle02:38

Mitogens and the Cell Cycle

Mitogens and their receptors play a crucial role in controlling the progression of the cell cycle. However, the loss of mitogenic control over cell division leads to tumor formation. Therefore, mitogens and mitogen receptors play an important role in cancer research. For instance, the epidermal growth factor (EGF) - a type of mitogen and its transmembrane receptor (EGFR), decides the fate of the cell's proliferation. When EGF binds to EGFR, a member of the ErbB family of tyrosine kinase...
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Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the daughter...
Receptor Downregulation in MVBs01:15

Receptor Downregulation in MVBs

Multivesicular bodies (MVBs) are mature endosomes that sort ubiquitinated proteins and then fuse with lysosomes to degrade the sorted proteins. Epidermal growth factor (EGF) and its receptor (EGFR) form a complex that can be internalized through endocytosis, sorted into an MVB, and later degraded.
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Interactions Between Signaling Pathways01:19

Interactions Between Signaling Pathways

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PI3K/mTOR/AKT Signaling Pathway01:22

PI3K/mTOR/AKT Signaling Pathway

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TGF - β Signaling Pathway01:16

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Molecular Analysis of Endothelial-mesenchymal Transition Induced by Transforming Growth Factor-β Signaling
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TGF-βシグナル伝達におけるサイトプラズミックPML機能

Hui-Kuan Lin1, Stephan Bergmann, Pier Paolo Pandolfi

  • 1Cancer Biology and Genetics Program, Department of Pathology, Memorial Sloan-Kettering Cancer Center, Sloan-Kettering Institute, 1275 York Avenue, New York, New York, 10021, USA.

Nature
|September 10, 2004
PubMed
まとめ
この要約は機械生成です。

サイトプラズマ性プロミエロサイト性白血病 (PML) タンパク質は,成長因子ベータ (TGF-β) 信号伝達の変換を調節する. PMLは,TGF-β誘発の成長停止とアポトーシスに不可欠であり,その欠如はSmadタンパク質の機能を損なう.

さらに関連する動画

Studying TGF-β Signaling and TGF-β-induced Epithelial-to-mesenchymal Transition in Breast Cancer and Normal Cells
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Visualization and Quantification of TGFβ/BMP/SMAD Signaling under Different Fluid Shear Stress Conditions using Proximity-Ligation-Assay
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Visualization and Quantification of TGFβ/BMP/SMAD Signaling under Different Fluid Shear Stress Conditions using Proximity-Ligation-Assay

Published on: September 14, 2021

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07:49

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06:54

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Visualization and Quantification of TGFβ/BMP/SMAD Signaling under Different Fluid Shear Stress Conditions using Proximity-Ligation-Assay
11:38

Visualization and Quantification of TGFβ/BMP/SMAD Signaling under Different Fluid Shear Stress Conditions using Proximity-Ligation-Assay

Published on: September 14, 2021

科学分野:

  • 細胞生物学 細胞生物学
  • 分子生物学は分子生物学である.
  • がん研究 がん研究

背景:

  • 変形成長因子β (TGF-β) は重要な腫瘍抑制剤であるが,がん細胞はしばしばその効果を回避する.
  • 腫瘍抑制剤として知られるプロミエロサイト性白血病 (PML) タンパク質には,異なる機能を持つ核および細胞プラズマの同型があります.
  • TGF-βシグナル伝達における細胞質PMLの役割は,ほとんど知られていなかった.

研究 の 目的:

  • TGF-βシグナル伝達経路における細胞質PMLの機能を調査する.
  • 細胞質PMLがTGF-β誘発細胞応答を調節するかどうかを判断する.
  • PMLがTGF-βシグナル伝達成分と相互作用する分子メカニズムを解明する.

主な方法:

  • TGF-β応答を評価するために,Pml-nullプライマリ細胞を使用した.
  • Smad2およびSmad3タンパク質のリン酸化と核転位を分析した.
  • PML,Smad2/3,SARA,TGF-β受容体間の物理的な相互作用を共免疫プレシピテーションおよび細胞局所化研究を使用して調査しました.

主要な成果:

  • Pml-null細胞は,TGF-β媒介の成長停止,衰老,アポトーシスに対する抵抗を示した.
  • Pml-null細胞では,Smad2/3の酸化と核転移の障害が観察されました.
  • 細胞質PMLはSmad2/3とSARAと物理的に相互作用し,Smad2/3-SARA複合体の形成と初期エンドソームにおける受容体の蓄積を促進する.
  • TGF-β治療は,細胞質PML発現を誘発した.
  • PML-RARアルファ・オンコタンパク質は,APL細胞のTGF-βシグナル伝達欠陥を反映した,細胞質PML機能に敵対する.

結論:

  • 細胞質PMLは,TGF-βシグナル伝達の重要な調節体であり,その腫瘍抑制機能を媒介するのに不可欠です.
  • PMLのSmadタンパク質と受容体トラフィックの相互作用は,TGF-β経路の有効な活性化に不可欠です.
  • 細胞質PMLとTGF-βシグナル伝達の制御不良は,特にAPLにおいて,がんの病原化に寄与する.