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Updated: Jul 18, 2026

A Novel Saturation Mutagenesis Approach: Single Step Characterization of Regulatory Protein Binding Sites in RNA Using Phosphorothioates
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RNA Diels-Alderasesに対する突然変異の効果

Theodore M Tarasow1, Elizabeth Kellogg, Benjamin L Holley

  • 1Department of Chemistry, College of Physical and Mathematical Sciences, North Carolina State University, Raleigh, North Carolina 27695-8204, USA.

Journal of the American Chemical Society
|September 24, 2004
PubMed
まとめ

エンジニアリングされたRNA Diels-Alderases (DA22) は,突然変異とインビトロ選択により,触媒効率が向上しています. これらの改良された酵素は特異性を維持し,1つの変種は新しい金属依存性を示しています.

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