ミオグロビンから一酸化炭素が,生理学的温度で溶液に脱出する過程
Yasutaka Nishihara1, Masaaki Sakakura, Yoshifumi Kimura
1Department of Chemistry, Graduate School of Science, Kyoto University, Kyoto, 606-8502, Japan.
Journal of the American Chemical Society
|September 24, 2004
まとめ
一酸化炭素 (CO) は,クセノン (Xe) の影響を受け,特定の部位を通ってミオグロビンから脱出します. Xe圧力は,COの脱出率に影響し,溶液に放出される前に,Xe (1) 部位でCOが閉じ込められていることを明らかにします.
科学分野:
- バイオフィジックス 生物物理学
- 物理化学 物理化学
背景:
- マイオグルビン (Mb) は,酸素を結合して貯蔵するタンパク質です.
- 一酸化炭素 (CO) は,Mbのヘム鉄と結合するリガンドです.
- リンガンド脱出メカニズムの理解は,タンパク質機能の研究に不可欠です.
研究 の 目的:
- ミオグロビン (Mb) から脱出する一酸化炭素 (CO) リガンド脱出経路を調査する.
- CO脱出ダイナミクスを調節するクセノン (Xe) の役割を明らかにする.
- リガンドの放出に関与する特定のドッキングサイトを特定するために.
主な方法:
- トランジエント・グリッティング (TG) 技術は,分子体積の変化を測定するために使用されました.
- 研究は生理学的温度で実施された.
- COの脱出率に及ぼす影響を観察するために,異なるクセノン圧力を適用しました.
主要な成果:
- 表面的なCOの脱出速度と体積収縮は,クセノン圧力とともに増加した.
- 圧力依存性は,Xe) 1) 場所のクセノン集団と相関しています.
- COは,溶媒に逃げる前にXE (1) 部位に閉じ込められていることが判明しました.
結論:
- A Xe(1) 捕獲部位は,Mb.から排出されるCOの排出に影響する.
- フォトエキシテーションは,XEに閉じ込められたMbCOから,閉じ込められたXEではなく,COを放出します.
- 2つの異なるCO脱出経路が存在し,一つは支配的でXE1) に依存し,もう一つはXEがXE1) サイトをブロックするときに利用されます.
さらに関連する動画
08:31Anaerobic Protein Purification and Kinetic Analysis via Oxygen Electrode for Studying DesB Dioxygenase Activity and Inhibition
Published on: October 3, 2018
06:12Mechanical Separation and Protein Solubilization of the Outer and Inner Perivitelline Sublayers from Hen's Eggs
Published on: January 27, 2021
関連する概念動画
Gas Exchange and Transport
Gas exchange, the intake of molecular oxygen (O2) from the environment and the outflow of carbon dioxide (CO2) into the environment, is necessary for cellular function. Gas exchange during respiration occurs largely via the movement of gas molecules along pressure gradients. Gas travels from areas of higher partial pressure to areas of lower partial pressure. In mammals, gas exchange occurs in the alveoli of the lungs, which are adjacent to capillaries and share a membrane with them.
The Carbon Cycle
Carbon is the basis of all organic matter on Earth, and is recycled through the ecosystem in two primary processes: one in which carbon is exchanged among living organisms, and one in which carbon is cycled over long periods of time through fossilized organic remains, weathering of rocks, and volcanic activity. Human activities, including increased agricultural practices and the burning of fossil fuels, has greatly affected the balance of the natural carbon cycle.
Protein Denaturation
The function of proteins depends on their native three-dimensional structure, which is dictated by the amino acid sequence of the specific protein. Folding of the polypeptide chain takes place under specific conditions that energetically favor the folded conformation. In contrast, protein denaturation occurs spontaneously under unfavorable conditions that disrupt the integrity of the folded conformation. Thus, the chemical and physical environment of a protein, such as significant changes in pH...
Oxygen Transport in the Blood
Hemoglobin (Hb) is a crucial molecule in the human body, consisting of four polypeptide chains, each bound to an iron-containing heme group. This unique structure enables hemoglobin to bind to oxygen, with each molecule capable of combining with four molecules of oxygen, leading to rapid and reversible oxygen loading. When fully loaded with oxygen, it is called oxyhemoglobin, while hemoglobin that has released oxygen is called reduced hemoglobin or deoxyhemoglobin. As hemoglobin binds oxygen,...
Carbon Dioxide Transport in the Blood
Carbon dioxide (CO2) transport in the blood is critical to human physiology. On average, our body cells produce around 200 mL of CO2 per minute, precisely the quantity expelled by the lungs. This process involves the transportation of CO2 from the tissue cells to the lungs in three primary forms.
Forms of CO2 Transport
1. Dissolved in plasma: A small percentage (7-10%) of CO2 is transported and dissolved directly in the plasma.
2. Carbaminohemoglobin: Just over 20% of CO2 is chemically bound to...
Forms of CO2 Transport
1. Dissolved in plasma: A small percentage (7-10%) of CO2 is transported and dissolved directly in the plasma.
2. Carbaminohemoglobin: Just over 20% of CO2 is chemically bound to...
Oxygenic Photosynthesis
Oxygenic photosynthesis is a fundamental process in which light energy is harnessed to drive the oxidation of water, leading to the production of molecular oxygen (O₂), adenosine triphosphate (ATP), and nicotinamide adenine dinucleotide phosphate (NADPH). This process is essential for sustaining aerobic life on Earth and is primarily carried out by cyanobacteria, algae, and plants. The core of oxygenic photosynthesis lies in the thylakoid membranes, where chlorophyll pigments facilitate light...
