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Updated: Jul 19, 2026
13:51
The MultiBac Protein Complex Production Platform at the EMBL
Published on: July 11, 2013
酵母遺伝子 (BEM1) は,細胞の極化に必要であり,その産物には2つのSH3ドメインが含まれています
J Chenevert1, K Corrado, A Bender
1Department of Biochemistry and Biophysics, University of California, San Francisco 94143.
Nature
|March 5, 1992
まとめ
研究者らは,Saccharomyces cerevisiaeの特定のBEM1遺伝子変異を特定し,交配中に細胞の偏振を妨げている. これらのBEM1-s変異体はBEM1を強調しています.
科学分野:
- 細胞生物学 細胞生物学
- 分子遺伝学 分子遺伝学
- バイオケミストリー バイオケミストリー
背景:
- 細胞の分極化は,細胞軸と細胞骨格組織の確立を含む細胞機能にとって極めて重要です.
- 細胞骨格と選択された細胞軸または部位を結びつける正確なメカニズムは,まだ完全に理解されていません.
- Saccharomyces cerevisiaeは,植物的成長と交配の両方で細胞の偏分を示し,異なる細胞内および細胞外信号に反応します.
研究 の 目的:
- 細胞外交フェロモンによる細胞外交への反応として,細胞の二極化の分子基礎を調査する.
- 酵母におけるフェロモン誘発の細胞極化に不可欠な遺伝子を特定し,特徴づけること.
主な方法:
- ペロモンの交配反応に特異的な欠陥がある酵母変異体の分離と特徴付け.
- BEM1遺伝子の遺伝子解析と細胞の偏極化におけるその役割.
- 機能ドメインを予測するためのBEM1タンパク質 (Bem1p) の配列分析.
主要な成果:
- 2つの新型変異体 (bem1-s) は,植物的成長ではなく,特に交配時に細胞の偏向に欠陥があることが確認されました.
- これらの変異体は,植物性細胞の極化に不可欠なBEM1遺伝子の特殊アレルを持ちます.
- BEM1タンパク質 (Bem1p) には,SH3ドメインが含まれており,細胞骨格と細胞表面の構成要素を結びつける役割が示唆されています.
結論:
- BEM1遺伝子は,植物的成長と交配の両方において,細胞の二極化において,重要な二重の役割を果たします.
- BEM1 (bem1-s) の特定のアレルは,細胞内信号と細胞外信号に対する偏振反応に差異的に影響する.
- Bem1pは分子ブリッジとして作用し,分極化時に細胞骨格と細胞表面の決定因子を結びつける.
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