基礎転写因子のプロモーター特異性
J D Parvin1, H T Timmers, P A Sharp
1Center for Cancer Research, Massachusetts Institute of Technology, Cambridge 02138.
Cell
|March 20, 1992
まとめ
すべての基礎転写因子が一般的ではない. アデノウイルスの主要遅発プロモーターのような一部のプロモーターは,基礎転写のために追加の因子 (TFIIE) と刺激タンパク質を必要とし,以前の仮定に異議を唱える.
科学分野:
- 分子生物学は分子生物学である.
- 遺伝子規制 遺伝子規制
- ユカリオットのトランスクリプション
背景:
- ユカリオットの遺伝子発現は,基礎因子とRNAポリメラーゼIIを調節する転写因子によって制御されます.
- ベース要因は以前,すべてのRNAポリメラーゼIIプロモーターに普遍的に不可欠であると考えられていた.
研究 の 目的:
- 特定のエウカリオットプロモーターからの転写のための基礎因子要件を調査する.
- 普遍的に不可欠な基礎因子のパラダイムがすべてのプロモーターに対して当てはまるかどうかを判断する.
主な方法:
- 精製された転写因子と特定のコアプロモーター (免疫グロブリン重鎖遺伝子とアデノウイルスメジャーラット遺伝子) を使用した基礎転写アッセイの復元.
- 特定の基礎転写因子 (TFIID,TFIIB,TFIIF,TFIIE) と追加のタンパク質活動に対する要求の分析.
主要な成果:
- 免疫グロブリン重鎖プロモーターの基礎転写には,TFIID,TFIIB,TFIIF,およびRNAポリメラーゼIIが必要でした.
- アデノウイルスの主要遅発プロモーターの基礎転写にはさらにTFIIEが必要であり,2つの新しいタンパク質活動 (700kdおよび90kd) によって刺激された.
結論:
- すべてのRNAポリメラーゼIIプロモーターに,すべての基礎転写因子が普遍的に必要とされるというパラダイムは,過度に単純化している.
- 特定のプロモーターは,より複雑な規制状況を示唆する,明確なベースファクター要件を示します.
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