大量スペクトロメトリによる環境条件下でのサンプリングは,脱吸収用エレクトロスプレーのイオン化によるものです
Zoltán Takáts1, Justin M Wiseman, Bogdan Gologan
1Department of Chemistry, Purdue University, West Lafayette, IN 47907, USA.
Science (New York, N.Y.)
|October 16, 2004
まとめ
新しく開発された脱吸収電スプレーイオン化 (DESI) 技術は,様々な表面上の様々な化合物の直接分析を可能にします. この方法は,従来の電気スプレーイオン化に匹敵する質量スペクトルを生成し,in vivo研究を容易にする.
科学分野:
- アナリティカル・ケミストリー (Analytical Chemistry) とは
- 表面科学とは,地表科学である.
- マススペクトロメトリーによる質量スペクトロメトリーです.
背景:
- 伝統的な質量スペクトロメトリー技術は,しばしばサンプル準備を必要とします.
- 表面に直接化合物を分析することは,分析的な課題を提示します.
- 電気スプレーイオン化 (ESI) は強力な技術ですが,通常は溶液中のサンプルが必要です.
研究 の 目的:
- 新しい脱吸収イオン化方法を導入し,特徴づけること.
- 異なる表面で様々な化合物を分析するためのこの方法の適用性を実証する.
- このテクニックを用いてin vivo分析の可能性を探求する.
主な方法:
- デソルプション・エレクトロスプレー・イオン化 (DESI) を採用した.
- 充電された溶媒の滴やイオンをサンプル表面に向けました.
- 結果となるガスイオンの分析は,質量スペクトロメトリを用いて行われました.
主要な成果:
- DESIは,非極性小分子,極性化合物,ペプチド,タンパク質を含む様々な化合物を成功裏にイオン化しました.
- このテクニックは,導電性および絶縁性表面 (金属,ポリマー,鉱物) に有効でした.
- 生成された質量スペクトルは,従来のESIの質量スペクトルと似ており,分子イオンを示しています.
結論:
- DESIは,表面分析のための多用途の脱吸収イオン化技術です.
- この方法は,生物学的サンプルを含む複雑なマトリックスにおける分析物の直接分析を可能にします.
- DESIは,in vivoの応用と,様々な基板上の化合物の分析に有望である.
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