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関連する概念動画

Intralumenal Vesicles and Multivesicular Bodies01:38

Intralumenal Vesicles and Multivesicular Bodies

Intraluminal vesicles (ILVs) are small vesicles 50-80 nm in diameter formed during the maturation of early endosomes. A specialized endosome containing numerous ILVs is called a multivesicular body (MVB). ILVs contain internalized molecules such as antigens, nucleic acids, proteins, and metabolites. Some of these molecules are released from the MVBs inside exosomes and are transported to other cells. Other MVBs contain molecules that are retained in the ILVs and are later degraded within the...
Overview of Secretory Vesicles01:33

Overview of Secretory Vesicles

Secretory vesicles, also known as dense core vesicles (DCVs), are membrane-bound vesicles that transport secretory proteins, such as hormones or neurotransmitters. Regulated secretory vesicles transport proteins from the trans-Golgi network to the exterior of the cell. Proteins present in regulated secretory vesicles are required to be rapidly exocytosed in large amounts upon a specific stimulus.
Various proteins regulate the aggregation of molecules inside the secretory vesicles. Chromogranins...
Fusion of Secretory Vesicles with the Plasma Membrane01:26

Fusion of Secretory Vesicles with the Plasma Membrane

Proteins and neurotransmitters in secretory vesicles can be released from a cell upon vesicle docking, priming, and fusion with the plasma membrane. Vesicles are docked and primed in preparation for the quick exocytosis of their contents in response to a stimulus. The fusion process is mainly carried out by a SNAP Receptor or SNARE complex, consisting of synaptobrevin, syntaxin-1, and SNAP-25.
In 1993, Jim Rothman proposed that the antiparallel pairing of vesicular and transmembrane SNAREs, or...
SNAREs and Membrane Fusion01:43

SNAREs and Membrane Fusion

Once a transport vesicle has recognized its target organelle, the vesicular membrane needs to fuse with the target membrane to unload the cargo. Transmembrane proteins called SNAREs present on organelle membranes and their vesicles, mediate vesicle fusion.
SNAREs exist in pairs that symmetrically interact and catalyze the fusion of the lipid bilayers in vesicle and target organelle. v-SNARE in the vesicle membrane are single polypeptide chains that bind to a complementary t-SNARE, composed of 2...
Vesicular Tubular Clusters01:45

Vesicular Tubular Clusters

After budding out from the ER membrane, some COPII vesicles lose their coat and fuse with one another to form larger vesicles and interconnected tubules called vesicular tubular clusters or VTCs. These clusters constitute a compartment at the ER-Golgi interface known as ERGIC (Endoplasmic Reticulum Golgi Intermediate Compartment). The ERGIC is a mobile membrane-bound cargo transport system that sorts proteins secreted from ER and delivers them to the Golgi.
With the help of motor proteins such...
Cotranslational Protein Translocation01:20

Cotranslational Protein Translocation

Translocation of proteins across membranes is an ancient process that occurs even in bacteria and archaebacteria. In fact, the components of the translocation machinery are still conserved between prokaryotes and eukaryotes.
Sec61 channel partners for cotranslational translocation
During cotranslational translocation, the Sec61 channel partners with the signal recognition particle (SRP), the signal recognition particle receptor (SR), and the ribosomes to transport the nascent polypeptide chain...

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関連する実験動画

Updated: Jul 6, 2026

Analysis of SNARE-mediated Membrane Fusion Using an Enzymatic Cell Fusion Assay
09:19

Analysis of SNARE-mediated Membrane Fusion Using an Enzymatic Cell Fusion Assay

Published on: October 19, 2012

生体内分泌細胞におけるSNARE複合体の形成を追跡する.

Seong J An1, Wolfhard Almers

  • 1Vollum Institute L-474, Oregon Health Sciences University, 3181 Southwest Sam Jackson Park Road, Portland, OR 97201, USA.

Science (New York, N.Y.)
|November 6, 2004
PubMed
まとめ
この要約は機械生成です。

研究者らは,神経細胞におけるSNAREタンパク質複合体の形成を研究した. 彼らは,シントキシン-SNAP25複合体を発見し,それはエクソサイトーシス過程の前駆体であり,カルシウム流入で逆転的に組み立てられる可能性がある.

さらに関連する動画

SNARE-mediated Fusion of Single Proteoliposomes with Tethered Supported Bilayers in a Microfluidic Flow Cell Monitored by Polarized TIRF Microscopy
10:58

SNARE-mediated Fusion of Single Proteoliposomes with Tethered Supported Bilayers in a Microfluidic Flow Cell Monitored by Polarized TIRF Microscopy

Published on: August 24, 2016

Visualizing Intracellular SNARE Trafficking by Fluorescence Lifetime Imaging Microscopy
08:55

Visualizing Intracellular SNARE Trafficking by Fluorescence Lifetime Imaging Microscopy

Published on: December 29, 2017

関連する実験動画

Last Updated: Jul 6, 2026

Analysis of SNARE-mediated Membrane Fusion Using an Enzymatic Cell Fusion Assay
09:19

Analysis of SNARE-mediated Membrane Fusion Using an Enzymatic Cell Fusion Assay

Published on: October 19, 2012

SNARE-mediated Fusion of Single Proteoliposomes with Tethered Supported Bilayers in a Microfluidic Flow Cell Monitored by Polarized TIRF Microscopy
10:58

SNARE-mediated Fusion of Single Proteoliposomes with Tethered Supported Bilayers in a Microfluidic Flow Cell Monitored by Polarized TIRF Microscopy

Published on: August 24, 2016

Visualizing Intracellular SNARE Trafficking by Fluorescence Lifetime Imaging Microscopy
08:55

Visualizing Intracellular SNARE Trafficking by Fluorescence Lifetime Imaging Microscopy

Published on: December 29, 2017

科学分野:

  • 神経科学は神経科学である.
  • 分子生物学は分子生物学である.
  • 細胞生物学 細胞生物学

背景:

  • シンタキシン,SNAP25,シナプトブロヴィンを含むSNAREタンパク質は,神経細胞のエキソサイトーシスに不可欠です.
  • SNARE核複合体の形成に伴う正確なステップは,ほとんど不明のままです.
  • ニューロンのエクソサイトーシスは,神経伝達物質の放出のための基本的なプロセスです.

研究 の 目的:

  • SNARE複合体の形成のインビボステップを調査する.
  • エキソサイトーシス経路における潜在的な前駆体複合体を特定する.
  • SNARE複合体の組立におけるカルシウムの役割を理解するために.

主な方法:

  • 複合形成をリアルタイムで監視するためにSNAP25の光タグ付きバージョンを使用しました.
  • ニューロンのエクソサイトーシスを研究するためのモデルシステムとしてPC12細胞を採用した.
  • 特定の突然変異とカルシウム流入が複雑な組み立てに与える影響を調査した.

主要な成果:

  • 高アフィニティのシンタキシン-SNAP25複合体の証拠は,in vivoで観察されました.
  • この複雑な形成には,SNAP25のアミノ端末SNAREモチーフのみが必要でした.
  • シンタキシン-SNAP25複合体は,脱極化中にカルシウムが入ると逆転的に組み立てられる.

結論:

  • 特定されたシンタキシン-SNAP25複合体は,SNAREコア複合体の重要な前駆体である可能性があります.
  • この前駆体複合体は,おそらく,エクソサイトーシスのカルシウム依存のプライミングステップに参加しています.
  • これらの初期段階を理解することで,神経伝達物質の放出の調節に関する洞察が得られます.