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関連する概念動画

MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...
RNA Interference01:23

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...
siRNA - Small Interfering RNAs02:30

siRNA - Small Interfering RNAs

Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the ATP-dependent...
piRNA - Piwi-interacting RNAs02:57

piRNA - Piwi-interacting RNAs

PIWI-interacting RNAs, or piRNAs, are the most abundant short non-coding RNAs. More than 20,000 genes have been found in humans that code for piRNAs while only 2000 genes have been found for miRNAs. piRNAs can act at the transcriptional and post-transcriptional levels and have a vital role in silencing transposable elements present in germ cells. They are also involved in epigenetic silencing and activation. Previously, they were thought to function only in germ cells but new evidence suggests...
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...

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関連する実験動画

Updated: May 7, 2026

A Complete Pipeline for Isolating and Sequencing MicroRNAs, and Analyzing Them Using Open Source Tools
09:29

A Complete Pipeline for Isolating and Sequencing MicroRNAs, and Analyzing Them Using Open Source Tools

Published on: August 21, 2019

マイクロプロセッサ複合体によるプライマリマイクロRNAの処理

Ahmet M Denli1, Bastiaan B J Tops, Ronald H A Plasterk

  • 1Cold Spring Harbor Laboratory, Watson School of Biological Sciences, 1 Bungtown Road, Cold Spring Harbor, New York 11724, USA.

Nature
|November 9, 2004
PubMed
まとめ
この要約は機械生成です。

ドロシャとパシャを含むマイクロプロセッサ複合体は,マイクロRNA (miRNA) の成熟に不可欠です. パシャ・パシャは,パシャである.

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関連する実験動画

Last Updated: May 7, 2026

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09:29

A Complete Pipeline for Isolating and Sequencing MicroRNAs, and Analyzing Them Using Open Source Tools

Published on: August 21, 2019

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A Reporter Assay to Analyze Intronic microRNA Maturation in Mammalian Cells
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科学分野:

  • 分子生物学は分子生物学である.
  • 遺伝学 遺伝学とは
  • バイオケミストリー バイオケミストリー

背景:

  • マイクロRNA (miRNA) は,遺伝子発現の重要な転写後の調節体である.
  • 成熟したmiRNAは,2段階の経路でプライマリmiRNAトランスクリプト (pri-miRNA) から処理されます.
  • ドロシャはRNase III核酵素で,核内でプリミRNA処理を開始する.

研究 の 目的:

  • ドロシャを含むマイクロプロセッサ複合体のタンパク質成分を調査する.
  • miRNAの生物発生と機能におけるパシャ (ドロシャのパートナー) の役割を解明する.

主な方法:

  • マイクロプロセッサ複合体の生化学的特徴.
  • DrosophilaとCaenorhabditis elegansにおけるパシャ発現の抑制を含む機能的研究.
  • プリミRNAと成熟ミRNAのレベルを分析する.
  • レポーターアッセイとC. elegans.のフェノタイプ分析

主要な成果:

  • ドロシャは,マルチタンパク質複合体であるマイクロプロセッサの一部として機能します.
  • パシャは,二重鎖のRNA結合タンパク質であり,マイクロプロセッサ複合体の構成要素である.
  • パシャの枯渇または突然変異は,プリミRNAの処理を阻害し,成熟ミRNAのレベルを低下させ,遺伝子調節に影響します.

結論:

  • パシャはmiRNAの成熟に重要な役割を果たしています.
  • パシャを含むマイクロプロセッサ複合体は,miRNA媒介の遺伝子調節に不可欠である.
  • パシャのmiRNAバイオゲネシスの機能は,種を超えて保存されています.