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Conformations of Cyclohexane02:11

Conformations of Cyclohexane

Cyclohexane does not exist in a planar form due to the high angle and torsional strain it would experience in the planar structure. Instead, it adopts non-planar chair and boat conformations.
The chair form is the most stable and derives its name from its resemblance to the “easy chair.” In the chair conformation, two carbon atoms are arranged out-of-plane — one above and one below, minimizing the torsional strain. In the chair form, the bond angle is very close to the ideal tetrahedral value,...
Chair Conformation of Cyclohexane02:02

Chair Conformation of Cyclohexane

The chair conformation is the most stable form of cyclohexane due to the absence of angle and torsional strain. The absence of angle strain is a result of cyclohexane’s bond angle being very close to the ideal tetrahedral bond angle of 109.5° in its chair conformer. Similarly, the torsional strain is also absent owing to the perfectly staggered arrangement of bonds.
The hydrogen atoms linked to carbons are arranged in two different axial and equatorial orientations to achieve this staggered...
Cohesins02:20

Cohesins

Cohesin protein complexes are a molecular glue that holds two sister chromatids together. They play an important role both in mitosis and meiosis. In mitosis, all cohesin complexes present on the chromosomes are removed before the start of the anaphase stage.
Cohesin complexes in Meiotic Division
Meiosis involves two distinct rounds of chromosomal segregation and cell divisions— Meiosis I followed by Meiosis II – producing four daughter cells. Meiosis I includes the separation of homologous...
Single-Strand DNA Binding Proteins01:03

Single-Strand DNA Binding Proteins

For successful DNA replication, the unwinding of double-stranded DNA must be accompanied by stabilization and protection of the separated single strands of the DNA. This crucial task is performed by single-strand DNA-binding (SSB) proteins. They bind to the DNA in a sequence-independent manner, which means that the nitrogenous bases of the DNA need not be present in a specific order for binding of SSB proteins to it. The binding of SSB proteins straightens single-stranded DNA (ssDNA) and makes...
Spin–Spin Coupling: One-Bond Coupling01:17

Spin–Spin Coupling: One-Bond Coupling

Coupling interactions are strongest between NMR-active nuclei bonded to each other, where spin information can be transmitted directly through the pair of bonding electrons. While nuclei polarize their electrons to the opposite spins, the bonding electron pair has opposite spins. Configurations with antiparallel nuclear spins are expected to be lower in energy. When coupling makes antiparallel states more favorable, J is considered to have a positive value. The one-bond coupling constant, 1J,...
Spin–Spin Coupling: Two-Bond Coupling (Geminal Coupling)01:20

Spin–Spin Coupling: Two-Bond Coupling (Geminal Coupling)

Two NMR-active nuclei bonded to a central atom can be involved in geminal or two-bond coupling. Geminal coupling is commonly seen between diastereotopic protons in chiral molecules and unsymmetrical alkenes, among others.
The central atom need not be NMR-active because its electrons are affected by the electron polarization of the spin-active atoms. However, spin information is transmitted less effectively than in one-bond coupling, and 2J values are usually weaker than 1J values. The energy of...

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関連する実験動画

Updated: Jul 18, 2026

Combining X-Ray Crystallography with Small Angle X-Ray Scattering to Model Unstructured Regions of Nsa1 from S. Cerevisiae
09:15

Combining X-Ray Crystallography with Small Angle X-Ray Scattering to Model Unstructured Regions of Nsa1 from S. Cerevisiae

Published on: January 10, 2018

(S,S) -トランス・サイクロペンタン - 制限ペプチド核酸. 結合親和性と配列特異性を改善する一般的なバックボーン変更です.

Jonathan K Pokorski1, Mark A Witschi, Bethany L Purnell

  • 1Department of Chemistry, Northwestern University, Evanston, Illinois 60208, USA.

Journal of the American Chemical Society
|November 19, 2004
PubMed
まとめ

トランス・サイクロペンタン・ダイアミンを含む改変ペプチド核酸 (PNA) は,より高い結合親和性とDNAへの特異性を示しています. これらの新しいtcypPNAは,ゲノム分析プローブにとって有望である.

さらに関連する動画

Combining Non-reducing SDS-PAGE Analysis and Chemical Crosslinking to Detect Multimeric Complexes Stabilized by Disulfide Linkages in Mammalian Cells in Culture
09:37

Combining Non-reducing SDS-PAGE Analysis and Chemical Crosslinking to Detect Multimeric Complexes Stabilized by Disulfide Linkages in Mammalian Cells in Culture

Published on: May 2, 2019

Constructing Cyclic Peptides Using an On-Tether Sulfonium Center
07:11

Constructing Cyclic Peptides Using an On-Tether Sulfonium Center

Published on: September 28, 2022

関連する実験動画

Last Updated: Jul 18, 2026

Combining X-Ray Crystallography with Small Angle X-Ray Scattering to Model Unstructured Regions of Nsa1 from S. Cerevisiae
09:15

Combining X-Ray Crystallography with Small Angle X-Ray Scattering to Model Unstructured Regions of Nsa1 from S. Cerevisiae

Published on: January 10, 2018

Combining Non-reducing SDS-PAGE Analysis and Chemical Crosslinking to Detect Multimeric Complexes Stabilized by Disulfide Linkages in Mammalian Cells in Culture
09:37

Combining Non-reducing SDS-PAGE Analysis and Chemical Crosslinking to Detect Multimeric Complexes Stabilized by Disulfide Linkages in Mammalian Cells in Culture

Published on: May 2, 2019

Constructing Cyclic Peptides Using an On-Tether Sulfonium Center
07:11

Constructing Cyclic Peptides Using an On-Tether Sulfonium Center

Published on: September 28, 2022

科学分野:

  • バイオケミストリー バイオケミストリー
  • 分子生物学は分子生物学である.
  • 合成化学 合成化学とは

背景:

  • ペプチド核酸 (PNA) は中立の脊椎を持つDNAを真似するものです.
  • アミノエチルグリシンPNA (aegPNA) は一般的なPNAタイプである.
  • PNAの結合親和性と特異性を高めることは,アプリケーションにとって極めて重要です.

研究 の 目的:

  • (S,S) -トランス・サイクロペンタン・ダイアミン単位を組み込んだ新しいクラスのPNAを合成し,特徴づけること.
  • これらの改変がDNA結合特性に与える影響を評価する.
  • ゲノム解析用プローブとして,これらの改変PNAsの潜在能力を探求する.

主な方法:

  • (S,S) -トランス・サイクロペンタン・ダイアミン単位の数と位置が異なるPNA骨格の合成.
  • 混合塩基配列をPNAのバックボーンに組み込む.
  • 補完的なDNAに対する結合親和性と配列特異性の評価.

主要な成果:

  • エチレンダイアミンを (S,S) - トランス・サイクロペンタン・ダイアミン単位に置き換えると,PNA結合親和性が著しく増加した.
  • 補完的なDNAに対する強化された配列特異性が観察されました.
  • 新型tcypPNAsは,核酸探査アプリケーションに適性を示しました.

結論:

  • (S,S) -トランス・サイクロペンタン・ダイアミン単位を組み込むことは,PNAのパフォーマンスを改善するための成功した戦略を表しています.
  • tcypPNAは,ゲノム分析のための強化された特性を提供します.
  • この研究は,PNAベースの分子診断と研究のためのツールキットを拡張します.