関連する実験動画

Updated: May 12, 2026

Activation of Apoptosis by Cytoplasmic Microinjection of Cytochrome c

Activation of Apoptosis by Cytoplasmic Microinjection of Cytochrome c

Published on: June 29, 2011

c-mycタンパク質による線維芽細胞におけるアポトーシスの誘導

G I Evan¹, A H Wyllie, C S Gilbert

¹Imperial Cancer Research Fund Laboratories, London, England.

|April 3, 1992

まとめ

線維芽細胞におけるc-mycタンパク質の構成的発現は,制御されていない成長ではなく,アポトーシス,またはプログラムされた細胞死を引き起こす. より高いc-mycレベルは,この細胞死に対する感受性を高めます.

科学分野:

分子生物学は分子生物学である.
細胞生物学細胞生物学
がん研究がん研究

背景:

ラット-1線維芽細胞におけるc-myc腫瘍遺伝子の構成的発現は,低血清条件下での成長停止を防ぐ.
継続的な増殖信号にもかかわらず,これらの細胞は,重要な細胞死のために蓄積されません.

研究の目的:

規制解除されたc-myc発現を有する線維芽細胞で観察された細胞死亡のメカニズムを調査する.
c-mycのアポプトティック機能とその他の既知の細胞役割との関係を決定する.

主な方法:

血清剥奪下で構成的なc-myc発現を有するラット-1線維芽細胞における細胞死亡の分析.
アポトーシス誘導に関与するc-mycタンパク質領域の特徴.
細胞周期の様々な段階において,規制解除されたc-mycを有する細胞におけるアポトーシスの評価と,アストレス方法.

主要な成果:

c-mycを発現する線維芽細胞の細胞死はc-mycタンパク質に依存し,アポトーシスによって発生する.
アポトーシス誘導に必要なc-mycタンパク質の領域は,共変異,自己調節,分化抑制の領域と重複する.
c-mycタンパク質のレベルが高くなるのは,血清欠乏時にアポトーシスへの感受性の増加と相関しています.
規制解除されたc-myc発現は,さまざまな停止方法と細胞サイクルフェーズで,成長停止細胞でアポトーシスを引き起こす.

結論:

c-mycタンパク質は,アポトーシスを誘発する上で重要な役割を果たし,細胞運命を決定する重要な調節体として作用する.
c-mycのアポプトシス機能は,他の腫瘍発生性および調節性機能と密接に関連しています.
規制解除されたc-myc発現は,細胞サイクルチェックポイントを覆し,プログラムされた細胞死を誘発することができ,がんの発症における複雑な役割を示唆しています.

さらに関連する動画

Suppression of Pro-fibrotic Signaling Potentiates Factor-mediated Reprogramming of Mouse Embryonic Fibroblasts into Induced Cardiomyocytes

Suppression of Pro-fibrotic Signaling Potentiates Factor-mediated Reprogramming of Mouse Embryonic Fibroblasts into Induced Cardiomyocytes

Published on: June 3, 2018

Knockdown of FAM83A to Verify Its Role in Cervical Cancer Cell Growth and Cisplatin Sensitivity

Knockdown of FAM83A to Verify Its Role in Cervical Cancer Cell Growth and Cisplatin Sensitivity

Published on: February 9, 2024

関連する概念動画

Mitogens and the Cell Cycle

Mitogens and the Cell Cycle

Mitogens and their receptors play a crucial role in controlling the progression of the cell cycle. However, the loss of mitogenic control over cell division leads to tumor formation. Therefore, mitogens and mitogen receptors play an important role in cancer research. For instance, the epidermal growth factor (EGF) - a type of mitogen and its transmembrane receptor (EGFR), decides the fate of the cell's proliferation. When EGF binds to EGFR, a member of the ErbB family of tyrosine kinase...

Abnormal Proliferation

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the daughter...

The Extrinsic Apoptotic Pathway

The Extrinsic Apoptotic Pathway

The extrinsic apoptotic pathway is initiated when extracellular death-inducing signals, such as specific cytokines, activate the death receptors expressed on the cell surface. The immune cells involved in this pathway are natural killer cells (NK cells) and cytotoxic T-lymphocytes. NK cells are critical in innate immune response, while cytotoxic T-lymphocytes are associated with adaptive immune response. These cells recognize specific receptors expressed on the altered cells and activate...

The Intrinsic Apoptotic Pathway

The Intrinsic Apoptotic Pathway

Internal cellular stress, such as cellular injury or hypoxia, triggers intrinsic apoptosis. The B-cell lymphoma 2 (Bcl-2) family of proteins are the primary regulators of the intrinsic apoptotic pathway. For example, during DNA damage, checkpoint proteins, such as Ataxia Telangiectasia Mutated (ATM protein) and Checkpoints Factor-2 (Chk2) proteins, are activated. These proteins phosphorylate p53 which further activates pro-apoptotic proteins, such as Bax, Bak, PUMA, and Noxa, and inhibits...

MAPK Signaling Cascades

MAPK Signaling Cascades

Mitogen-activated protein kinase, or MAPK pathway, activates three sequential kinases to regulate cellular responses such as proliferation, differentiation, survival, and apoptosis. The canonical MAPK pathway starts with a mitogen or growth factor binding to an RTK. The activated RTKs stimulate Ras, which recruits Raf or MAP3 Kinase (MAPKKK), the first kinase of the MAPK signaling cascade. Raf further phosphorylates and activates MEK or MAP2 Kinases (MAPKK), which in turn phosphorylates MAP...

Induced Pluripotent Stem Cells

Induced Pluripotent Stem Cells

Stem cells are undifferentiated cells that divide and produce different cell types. Ordinarily, cells that have differentiated into a specific cell type are terminally differentiated; however, scientists have found a way to reprogram these mature cells so that they dedifferentiate and return to an unspecialized, proliferative state. These cells are pluripotent like embryonic stem cells—able to produce all cell types—and are called induced pluripotent stem cells (iPSCs).
Somatic cells are...