哺乳類の筋肉細胞は,その rostrocaudal 位置の細胞自律的,遺伝的な記憶を持っています
M J Donoghue1, R Morris-Valero, Y R Johnson
1Department of Anatomy and Neurobiology, Washington University School of Medicine, St. Louis, Missouri 63110.
Cell
|April 3, 1992
まとめ
筋肉細胞は位置記憶を保持し,クロランフェニコルアセチルトランスフェラーゼ (CAT) の発現率が高くなっている. この位置情報は,胚の発達中に確立され,成人期を通じて維持されます.
科学分野:
- 発達生物学 発達生物学とは
- 細胞生物学 細胞生物学
- 遺伝学 遺伝学とは
背景:
- クロランフェニコルアセチルトランスフェラーゼ (CAT) 発現の有意なロストロカウダル・グラディエントは,成人のマウスの筋肉に存在する.
- このグラデーションは,筋肉軸に沿った遺伝子発現の位置差を示している.
研究 の 目的:
- 筋肉細胞における位置情報の発達と維持を調査する.
- ミョボラストがポジショナルの記憶を保持しているかどうかを in vitro で判定する.
主な方法:
- 成人マウスの筋肉から細胞培養を確立する.
- 個々の成人の肌性細胞 (衛星細胞) からクローンを生成する.
- 胚性ミオブラストと腫瘍遺伝子の変異による衛星細胞系を培養する.
主要な成果:
- CATレベルは,原発筋のロストロカウダル位置を正確に反映した.
- ポジショナルの記憶は,成人の筋肉,胚性ミオブラスト,細胞系からの培養物で観察されました.
- この記憶は細胞自律的であり,遺伝可能であり,安定していた.
結論:
- ミオブラストは,胚形成中に確立された固有の位置記憶を持っています.
- この記憶は,成人細胞で維持され,変換に安定しています.
- クローン細胞系は,この位置記憶を研究するために貴重なモデルを提供します.
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