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Updated: Jul 6, 2026

Genetic and Biochemical Approaches for In Vivo and In Vitro Assessment of Protein Oligomerization: The Ryanodine Receptor Case Study
12:43

Genetic and Biochemical Approaches for In Vivo and In Vitro Assessment of Protein Oligomerization: The Ryanodine Receptor Case Study

Published on: July 27, 2016

タンパク質-リガンド結合親和性をランク付けし,化合物混合物におけるアロステリック対直接結合部位競争を決定する一般的な技術

D Allen Annis1, Naim Nazef, Cheng-Chi Chuang

  • 1NeoGenesis Pharmaceuticals Inc., 840 Memorial Drive, Cambridge, MA 02139, USA. aannis@neogenesis.com

Journal of the American Chemical Society
|November 26, 2004
PubMed
まとめ

私たちは,タンパク質-リガンド結合親和度をランク付けし,結合部位によってリガンドを分類するために,親和選択質量スペクトロメトリ (AS-MS) 方法を開発しました. このツールは,疾患標的の鉛化合物を効率的に特定し,最適化することで,薬剤発見に役立ちます.

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Resolving Affinity Purified Protein Complexes by Blue Native PAGE and Protein Correlation Profiling09:35

Resolving Affinity Purified Protein Complexes by Blue Native PAGE and Protein Correlation Profiling

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Combining Chemical Cross-linking and Mass Spectrometry of Intact Protein Complexes to Study the Architecture of Multi-subunit Protein Assemblies10:01

Combining Chemical Cross-linking and Mass Spectrometry of Intact Protein Complexes to Study the Architecture of Multi-subunit Protein Assemblies

Published on: November 28, 2017

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Protein-protein Interfaces02:04

Protein-protein Interfaces

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Protein Networks02:26

Protein Networks

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Protein Complexes with Interchangeable Parts

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Immunoprecipitation01:20

Immunoprecipitation

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Enzyme-Linked Immunosorbent Assay01:33

Enzyme-Linked Immunosorbent Assay

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There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen.  Enzyme-substrate reaction allows the antigen to be visualized or quantified.
Affinity Chromatography01:03

Affinity Chromatography

Affinity chromatography is a powerful technique extensively utilized for separating and purifying specific biomolecules from complex mixtures. It capitalizes on the highly selective binding between an analyte and its counterpart, such as antibody-antigen interactions. The counterpart is immobilized on the stationary phase, forming an affinity column. The stationary phase typically consists of solid support, such as agarose or porous glass beads, immobilizing the affinity ligand. The mobile...