Jove
Visualize
お問い合わせ
JoVE
x logofacebook logolinkedin logoyoutube logo
JoVEについて
概要リーダーシップブログJoVEヘルプセンター
著者向け
出版プロセス編集委員会範囲と方針査読よくある質問投稿
図書館員向け
推薦の声購読アクセスリソース図書館諮問委員会よくある質問
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experimentsアーカイブ
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教員リソースセンター教員サイト
利用規約
プライバシーポリシー
ポリシー

関連する概念動画

Transcription Factors02:16

Transcription Factors

Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
DNA-only Transposons02:57

DNA-only Transposons

DNA-only transposons are called autonomous transposons since they code for the enzyme transposase that is required for the transposition mechanism. Insertion of transposons can alter gene functions in multiple ways. They can mutate the gene, alter gene expression by introducing a novel promoter or insulator sequence, introduce new splice sites, and change the mRNA transcripts produced, or remodel chromatin structure.
The donor site from where the transposon is excised is either degraded or...
Transcription Factors02:16

Transcription Factors

Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
Master Transcription Regulators02:23

Master Transcription Regulators

Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a  complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
General Transcription Factors01:30

General Transcription Factors

Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
Master Transcription Regulators02:23

Master Transcription Regulators

Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a  complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...

こちらも読む

関連記事

共著者、ジャーナル、引用グラフによってこの研究に関連する記事。

並び替え
Same author

Preoperative Systemic Pan-Immune-Inflammation Value Predicts Posthepatectomy Liver Failure After Laparoscopic Resection for Hepatocellular Carcinoma: A Retrospective Cohort Study.

Journal of inflammation research·2026
Same author

Small Molecule Activators of Antitumor Immunity.

Accounts of chemical research·2026
Same author

Structural Control of Dual Emission in Coumarin Fluorophores for Visualizing Protein Droplet Maturation.

Angewandte Chemie (International ed. in English)·2026
Same author

National and Provincial Lifetime Risk of Chronic Respiratory Disease - China, 2023.

China CDC weekly·2026
Same author

National and regional lifetime risks of developing and dying from digestive system cancers in China: Findings of the Global Burden of Disease Study 2023.

Chinese medical journal·2026
Same author

Acellular Dermal Matrix-assisted Implant-based Breast Reconstruction: Surgical and Patient-reported Outcomes From the Mastectomy Reconstruction Outcomes Consortium Study.

Plastic and reconstructive surgery. Global open·2026

関連する実験動画

Updated: Jul 5, 2026

Describing a Transcription Factor Dependent Regulation of the MicroRNA Transcriptome
07:23

Describing a Transcription Factor Dependent Regulation of the MicroRNA Transcriptome

Published on: June 15, 2016

小分子転写因子を模倣する.

Youngjoo Kwon1, Hans-Dieter Arndt, Qian Mao

  • 1The Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, Texas 77030, USA.

Journal of the American Chemical Society
|December 9, 2004
PubMed
まとめ

研究者らは,転写因子を模倣する合成の非ペプチド分子を作成しました. この新しい化合物は,DNAとタンパク質複合体に結合することによって遺伝子発現を制御し,化学生物学における新しいアプローチを示しています.

科学分野:

  • 化学生物学 化学生物学とは
  • 分子生物学は分子生物学である.
  • 合成化学 合成化学とは

背景:

  • 転写因子は,真核生物学の基本的なプロセスである遺伝子発現を調節する.
  • 小さな有機分子を用いた転写因子の外部制御は,化学における重要な課題である.

研究 の 目的:

  • 転写因子の機能を模倣する完全に有機的,非ペプチド性化合物を設計,合成する.
  • このような合成分子が遺伝子発現を外部から制御できることを実証する.

主な方法:

  • DNA結合のためのヘアピンポリアミドと,ヒト媒介体複合体のSur-2サブユニットと結合するためのwrenchnololを組み合わせたハイブリッド分子を設計した.
  • レポーター遺伝子の転写を活性化する化合物の能力をin vitroでテストしました.

主要な成果:

  • ハイブリッド有機化合物は,プロモーターに依存した方法でレポーター遺伝子転写を成功裏に活性化しました.
  • 化合物は,DNAとSur-2タンパク質の両方との同時相互作用によってこの活性化を達成しました.

結論:

  • 転写因子として機能する合成非ペプチド分子を設計することは可能である.

さらに関連する動画

DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation
09:26

DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation

Published on: December 29, 2021

A Reporter Assay to Analyze Intronic microRNA Maturation in Mammalian Cells
06:48

A Reporter Assay to Analyze Intronic microRNA Maturation in Mammalian Cells

Published on: June 16, 2022

関連する実験動画

Last Updated: Jul 5, 2026

Describing a Transcription Factor Dependent Regulation of the MicroRNA Transcriptome
07:23

Describing a Transcription Factor Dependent Regulation of the MicroRNA Transcriptome

Published on: June 15, 2016

DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation
09:26

DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation

Published on: December 29, 2021

A Reporter Assay to Analyze Intronic microRNA Maturation in Mammalian Cells
06:48

A Reporter Assay to Analyze Intronic microRNA Maturation in Mammalian Cells

Published on: June 16, 2022

  • この研究は,設計された有機化合物を用いて遺伝子発現を制御するための新しい道を開きます.