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¹³C NMR: Distortionless Enhancement by Polarization Transfer (DEPT)01:20

¹³C NMR: Distortionless Enhancement by Polarization Transfer (DEPT)

When proton-coupled carbon-13 spectra are simplified by a broadband proton decoupling technique, structural information about the coupled protons is lost. Distortionless enhancement by polarization transfer (DEPT) is a technique that provides information on the number of hydrogens attached to each carbon in a molecule. While the DEPT experiment utilizes complex pulse sequences, the pulse delay and flip angle are specifically manipulated. The resulting signals have different phases depending on...
NMR Spectrometers: Resolution and Error Correction01:14

NMR Spectrometers: Resolution and Error Correction

When magnetic nuclei in a sample achieve resonance and undergo relaxation, the signal detected in NMR is an approximately exponential free induction decay. Fourier transform of an exponential decay yields a Lorentzian peak in the frequency domain. Lorentzian peaks in an NMR spectrum are defined by their amplitude, full width at half maximum, and position, where the peak width is governed by the spin-spin relaxation time alone. In real experiments, however, the applied magnetic field is rendered...
Applications Of NMR In Biology01:25

Applications Of NMR In Biology

Nuclear magnetic resonance (NMR) spectroscopy is a very valuable analytical technique for researchers. It has been used for more than 50 years as an analytical tool. F. Bloch and E. Purcell formulated NMR in 1946 and won the 1952 Nobel Prize in Physics  for their work. Biological macromolecules such as proteins, nucleic acids, lipids, and organic molecules including pharmaceutical compounds, can be studied using this versatile tool that exploits the magnetic properties of certain nuclei.
The...
Chemical Shift: Internal References and Solvent Effects01:17

Chemical Shift: Internal References and Solvent Effects

In an NMR sample, precise measurement of the absolute absorption frequencies of nuclei is difficult. A standard internal reference compound is added, and the frequency difference between the reference signal and sample signals is measured.
The internal reference compound generally used in NMR spectroscopy is tetramethylsilane (TMS). TMS is preferred because it is chemically inert, soluble in NMR solvents, and easily removable. Also, the highly shielded methyl protons in TMS yield an intense...
Insensitive Nuclei Enhanced by Polarization Transfer (INEPT)01:15

Insensitive Nuclei Enhanced by Polarization Transfer (INEPT)

Insensitive Nuclei Enhanced by Polarization Transfer (INEPT) is an advanced Nuclear Magnetic Resonance (NMR) technique specifically designed to detect and enhance the signals of low-abundance nuclei, such as carbon-13 and nitrogen-15, in small molecules. The fundamental principle behind INEPT is the transfer of polarization from a more abundant and highly polarizable nucleus, typically hydrogen-1, to the low-abundance nucleus of interest. This process effectively boosts the NMR signal of the...
Double Resonance Techniques: Overview01:12

Double Resonance Techniques: Overview

Double resonance techniques in Nuclear Magnetic Resonance (NMR) spectroscopy involve the simultaneous application of two different frequencies or radiofrequency pulses to manipulate and observe two distinct nuclear spins. One important application of double resonance is spin decoupling, which selectively suppresses coupling with one type of nucleus while observing the NMR signal from another nucleus, simplifying the spectrum and enhancing resolution.
Spin decoupling is usually achieved by...

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Atomic Scale Structural Studies of Macromolecular Assemblies by Solid-state Nuclear Magnetic Resonance Spectroscopy
14:55

Atomic Scale Structural Studies of Macromolecular Assemblies by Solid-state Nuclear Magnetic Resonance Spectroscopy

Published on: September 17, 2017

暗黙の溶媒と高度なサンプリング技術を使用して,NMR構造の精製.

Jianhan Chen1, Wonpil Im, Charles L Brooks

  • 1Department of Molecular Biology, Center for Theoretical Biological Physics, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, USA.

Journal of the American Chemical Society
|December 9, 2004
PubMed
まとめ
この要約は機械生成です。

暗黙の溶媒モデルは,特に限られた実験データでは,タンパク質のNMR構造の精製品質を大幅に改善します. これらのモデルとレプリカ交換 (REX) メソッドを組み合わせると,構造を元の状態に素早く導きます.

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The Identification of Sea Lamprey Pheromones Using Bioassay-Guided Fractionation
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15N CPMG Relaxation Dispersion for the Investigation of Protein Conformational Dynamics on the µs-ms Timescale
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Atomic Scale Structural Studies of Macromolecular Assemblies by Solid-state Nuclear Magnetic Resonance Spectroscopy
14:55

Atomic Scale Structural Studies of Macromolecular Assemblies by Solid-state Nuclear Magnetic Resonance Spectroscopy

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科学分野:

  • 構造生物学 構造生物学とは
  • コンピューティング・ケミストリー
  • バイオフィジックス 生物物理学

背景:

  • 核磁共鳴 (NMR) バイオ分子構造の計算は,形状サンプル採取のためのシミュレートアニリングに依存しています.
  • 実験的拘束装置の高冗長性は,通常,正確な3次元構造の決定のために必要である.
  • 一般化されたBorn (GB) 暗黙の溶媒モデルは,実験データを経験的力場と統合し,NMR構造を改善する可能性を秘めています.

研究 の 目的:

  • タンパク質のNMR構造の精製に対する暗黙の溶媒の影響を調査する.
  • 暗黙の溶媒モデルと先端のサンプリング技術を使用して,NMR構造の精製に最適なプロトコルを特定する.
  • 提案されたプロトコルの有効性を,異なるサイズとデータ冗長性のレベルのタンパク質にわたって評価する.

主な方法:

  • 構造精製実験は,NMR制約の完全およびサブセットを使用して,公開されたNMR構造を持つモデルタンパク質で行われました.
  • 暗黙の溶媒効果は,レプリカ交換 (REX) のような高度なサンプリング技術の適用とともに研究されました.
  • 従来のNMRソフトウェアによる初期構造生成と,暗黙の溶媒とREX.を使用した後の精錬を含む最適なプロトコルが開発されました.

主要な成果:

  • 暗黙の溶媒は,十分な実験的制約が利用可能な場合に精製に最小限の影響を及ぼしました.
  • 暗黙の溶媒による精製は,実験データが限られた場合に構造の質を大幅に改善しました.
  • 暗黙の溶媒とREXメソッドの組み合わせにより,ネイティブに近い構造がネイティブ盆地に向かって急速に導かれ,低エネルギー構造の強化されたサンプリングと自動選択が提供されました.

結論:

  • 実験データと初期構造生成を組み合わせ,暗黙の溶媒とREXを用いた後の精錬を行う最適なプロトコルは,特に制限が限られている場合に,NMR構造の質を大幅に改善します.
  • このプロトコルは,特に,NMR構造決定の初期段階において,また,大型多領域タンパク質や固体NMRなど,冗長データが限られているバイオ分子においては,特に有益である.
  • 提案された方法は,限られたデータからネイティブフォールドの信頼性の高い推定を提供することによって,全体的なNMR構造決定プロセスを加速します.