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Updated: Jul 10, 2026

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Hi-C: A Method to Study the Three-dimensional Architecture of Genomes.
Published on: May 6, 2010
鶏のゲノムの物理的な地図
John W Wallis1, Jan Aerts, Martien A M Groenen
1Genome Sequencing Center, Washington University School of Medicine, Campus Box 8501, 4444 Forest Park Avenue, St Louis, Missouri 63108, USA.
Nature
|December 14, 2004
まとめ
指紋クローンマッピングを使用して,鶏のゲノムの新しい物理的な地図が作成されました. この20倍カバーのマップは,ゲノムアセンブリと機能的ゲノム研究を検証するのに役立ちます.
科学分野:
- ゲノミクスゲノミクスとは
- 比較ゲノミクスとは
- バイオインフォマティックス
背景:
- ゲノム組み立て戦略は,全ゲノムショットガン配列と階層的な地図支援配列を組み合わせています.
- 細胞遺伝学から配列解像度までの全ゲノムマップは,正確なゲノムアセンブリにとって極めて重要です.
- 指紋クローンマップは,ゲノム配列の検証とアンカリングのために,オーダーされた重複するクローンを提供します.
研究 の 目的:
- 鶏のゲノム (Gallus gallus) のクローンベースの物理マップを提示する.
- チキンゲノム配列の草案を検証するためのリソースを提供し,機能的ゲノム研究を促進します.
- 鶏のクローンを他の配列化されたゲノムと並べて比較ゲノミクスを可能にします.
主な方法:
- 指紋技術を用いたクローンベースの物理マップの構築.
- 鶏のゲノムを表すクローンの制限酵素ダイジェストを利用する.
- 鶏のゲノムを重複するクローンで20倍にカバーする.
主要な成果:
- 260個の重複するクローンのコンティグを構成する物理的な地図が生成されました.
- この地図は,鶏のゲノムの約91%をカバーしています.
- 他の配列化されたゲノム内の位置に整合した鶏のクローンの識別が可能になりました.
結論:
- 提示された物理的な地図は,Gallus gallusのゲノムにとって貴重なリソースです.
- このマップは,ゲノムアセンブリの順序を検証し,配列のギャップを埋めるのに役立ちます.
- この地図は,将来の機能的ゲノム研究と比較分析を容易にする.
関連する概念動画
Organization of Genes
Overview
Organization of Genes
Overview
Forces Acting on Chromosomes
During mitosis, chromosome movements occur through the interplay of multiple piconewton level forces. In prometaphase, these forces help in chromosome assembly or congression at the equatorial plane, eventually leading to their alignment at the metaphase plate. The forces acting on the chromosomes are space and time-dependent; therefore, they vary with the position of the chromosomes as the cell progresses through mitosis.
Microtubules and motor proteins exert two types of forces on...
Microtubules and motor proteins exert two types of forces on...
Epistasis Analysis
Although Mendel chose seven unrelated traits in peas to study gene segregation, most traits involve multiple gene interactions that create a spectrum of phenotypes. When the interaction of various genes or alleles at different locations influences a phenotype, this is called epistasis. Epistasis often involves one gene masking or interfering with the expression of another (antagonistic epistasis). Epistasis often occurs when different genes are part of the same biochemical pathway. The...
Forces Acting on Chromosomes
During mitosis, chromosome movements occur through the interplay of multiple piconewton level forces. In prometaphase, these forces help in chromosome assembly or congression at the equatorial plane, eventually leading to their alignment at the metaphase plate. The forces acting on the chromosomes are space and time-dependent; therefore, they vary with the position of the chromosomes as the cell progresses through mitosis.
Microtubules and motor proteins exert two types of forces on...
Microtubules and motor proteins exert two types of forces on...
Genetic Material
Within the human body, a complex and detailed system of trillions of cells works in unison to sustain life. Each cell houses a nucleus, which contains 46 chromosomes divided into 23 pairs. Chromosomes are highly coiled structures made of the genetic material DNA. These chromosomes are essential carriers of genetic information, with half inherited from the mother through her egg and the other half from the father's sperm, combining to create the unique genetic makeup of an individual.

