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関連する概念動画

Bacterial RNA Polymerase00:43

Bacterial RNA Polymerase

Unlike eukaryotes, bacteria use a single RNA Polymerase (RNAP) to transcribe all genes. The different subunits of bacterial RNAPhave distinct functions. The multisubunit structure of the bacterial RNAP helps the enzyme to maintain catalytic function, facilitate assembly, interact with DNA and RNA, and self-regulate its activity.
In most genes, the transcription site is a single base present upstream of the coding sequence. Though RNAP is a catalytically efficient enzyme, it does not recognize...
RNA Polymerase II Accessory Proteins02:36

RNA Polymerase II Accessory Proteins

Proteins that regulate transcription can do so either via direct contact with RNA Polymerase or through indirect interactions facilitated by adaptors, mediators, histone-modifying proteins, and nucleosome remodelers. Direct interactions to activate transcription is seen in bacteria as well as in some eukaryotic genes. In these cases, upstream activation sequences are adjacent to the promoters, and the activator proteins interact directly with the transcriptional machinery. For example, in...
Ribosomal RNA Synthesis02:53

Ribosomal RNA Synthesis

Ribosome synthesis is a highly complex and coordinated process involving more than 200 assembly factors. The synthesis and processing of ribosomal components occurs not only in the nucleolus but also in the nucleoplasm and the cytoplasm of eukaryotic cells.
Ribosome biogenesis begins with the synthesis of 5S and 45S pre-rRNAs by distinct RNA polymerases. The primary transcripts are extensively processed and modified before they are bound and folded by ribosomal proteins and assembly factors,...
Cooperative Binding of Transcription Regulators02:13

Cooperative Binding of Transcription Regulators

Transcriptional regulators bind to specific cis-regulatory sequences in the DNA to regulate gene transcription. These cis-regulatory sequences are very short, usually less than ten nucleotide pairs in length. The short length means that there is a high probability of the exact same sequence randomly occurring throughout the genome.  Since regulators can also bind to groups of similar sequences, this further increases the chances of random binding. Transcriptional regulators form dimers that...
piRNA - Piwi-interacting RNAs02:57

piRNA - Piwi-interacting RNAs

PIWI-interacting RNAs, or piRNAs, are the most abundant short non-coding RNAs. More than 20,000 genes have been found in humans that code for piRNAs while only 2000 genes have been found for miRNAs. piRNAs can act at the transcriptional and post-transcriptional levels and have a vital role in silencing transposable elements present in germ cells. They are also involved in epigenetic silencing and activation. Previously, they were thought to function only in germ cells but new evidence suggests...
RNA Polymerase II Accessory Proteins02:36

RNA Polymerase II Accessory Proteins

Proteins that regulate transcription can do so either via direct contact with RNA Polymerase or through indirect interactions facilitated by adaptors, mediators, histone-modifying proteins, and nucleosome remodelers. Direct interactions to activate transcription is seen in bacteria as well as in some eukaryotic genes. In these cases, upstream activation sequences are adjacent to the promoters, and the activator proteins interact directly with the transcriptional machinery. For example, in...

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関連する実験動画

Updated: Jul 8, 2026

Isolation of Cognate RNA-protein Complexes from Cells Using Oligonucleotide-directed Elution
10:53

Isolation of Cognate RNA-protein Complexes from Cells Using Oligonucleotide-directed Elution

Published on: January 16, 2017

2つのRNAi複合体であるRITSとRDRCは,物理的に相互作用し,非コーディングセンターメリックRNAに局所化する.

Mohammad R Motamedi1, André Verdel, Serafin U Colmenares

  • 1Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA.

Cell
|December 21, 2004
PubMed
まとめ

RNA誘導RNAポリメラーゼ複合体 (RDRC) は,RNA誘導トランスクリプションサイレンシング (RITS) 複合体と物理的に,機能的に結合する. この相互作用は,分裂酵母におけるsiRNAに依存したヘテロクロマチン組成に極めて重要です.

さらに関連する動画

Novel RNA-Binding Proteins Isolation by the RaPID Methodology
11:19

Novel RNA-Binding Proteins Isolation by the RaPID Methodology

Published on: September 30, 2016

RNA-Associated Chromatin DNA-DNA Interaction Method
11:01

RNA-Associated Chromatin DNA-DNA Interaction Method

Published on: April 30, 2026

関連する実験動画

Last Updated: Jul 8, 2026

Isolation of Cognate RNA-protein Complexes from Cells Using Oligonucleotide-directed Elution
10:53

Isolation of Cognate RNA-protein Complexes from Cells Using Oligonucleotide-directed Elution

Published on: January 16, 2017

Novel RNA-Binding Proteins Isolation by the RaPID Methodology
11:19

Novel RNA-Binding Proteins Isolation by the RaPID Methodology

Published on: September 30, 2016

RNA-Associated Chromatin DNA-DNA Interaction Method
11:01

RNA-Associated Chromatin DNA-DNA Interaction Method

Published on: April 30, 2026

科学分野:

  • エピジェネティクス エピジェネティクス
  • RNA干渉 (RNAi) とは
  • 分子生物学は分子生物学である.

背景:

  • RNAi媒介によるヘテロクロマチンの組み立てには,RITS複合体とRdp1.1が必要です.
  • この過程におけるRdp1の正確な機能と相互作用は完全に理解されていません.

研究 の 目的:

  • ヘテロクロマチン組立におけるRdp1の分子相互作用と機能的重要性を明らかにする.
  • Rdp1とその関連タンパク質によって形成された複合体を特徴付ける.

主な方法:

  • 相互作用するタンパク質複合体を識別するための共免疫プレシピテーション.
  • 顕微鏡を用いたタンパク質の局所化分析.
  • ノックアウト株におけるsiRNAプロファイリング.
  • RNA指向のRNAポリメラーゼ活性に対する測定.

主要な成果:

  • Rdp1はHrr1 (RNAヘリアゼ) とCid12 (polyAポリマーゼ) と複合体 (RDRC) を形成し,RNA指向のRNAポリマーゼ活性を持つ.
  • RDRCはRITSと相互作用し,Dicer (Dcr1) と Clr4.4 を必要とする.
  • RDRCとRITSはどちらも核に局所化し,Dcr1に依存した方法でセンターメリックRNAと結合する.
  • Rdp1,Hrr1,またはCid12の喪失は,RITSにsiRNAsが欠け,セントロメリックの繰り返しに局所化できなくなります.

結論:

  • Rdp1とRITS複合体との間に物理的,機能的なリンクが存在します.
  • 非コーディングRNAは,特定のゲノム領域へのRNAi機械のsiRNA依存の局所化のためのプラットフォームとして機能します.
  • この研究は,RNA指向RNAポリメラーゼ活性とRITSによって媒介されるヘテロクロマチン形成のための新しいメカニズムを明らかにしています.