細胞粘着を調節する遺伝子のクローニングと特徴づけ
1Imperial Cancer Research Fund, Lincoln's Inn Fields, London, UK.
Nature
|April 9, 1992
まとめ
研究者らは,細胞外マトリックスへの細胞粘着を強化する細胞粘着レギュレータ (CAR) という新しい遺伝子を特定しました. この発見は,細胞粘着シグナル伝達経路をターゲットにすることで,腫瘍の侵入を抑制するための新しい戦略を提供する可能性があります.
科学分野:
- 分子生物学は分子生物学である.
- 細胞生物学 細胞生物学
- 腫瘍学 腫瘍学
背景:
- カデリンとインテグリン族は,細胞-細胞と細胞-マトリックス結合を媒介し,上皮質の分化,発がん,転移に影響を与えます.
- 結腸がん細胞は,コラーゲン1型に活発に結合し,インテグリン媒介の腺分化を引き起こします.
研究 の 目的:
- 大腸がん細胞におけるインテグリン機能の調節を調査する.
- 細胞-マトリックス結合に関与する分子を特定し,がんの進行におけるそれらの役割.
主な方法:
- モノクローナル抗体選択を用いた修飾された哺乳類発現クローンシステムを採用した.
- 選択は,コラーゲン1型に結合する細胞の粘着性フェノタイプに基づいて行われました.
主要な成果:
- 補完的なDNAクローンである細胞粘着調節器 (CAR) が分離され,細胞外マトリックス成分に対する細胞粘着を強める.
- 16q染色体にあるCAR遺伝子は142アミノ酸タンパク質をコードし,N末端のミリスチル化モチーフとC末端のチロシンキナーゼリン酸化部位を有する.
- C端のチロシン残基の除去により,細胞-マトリックス粘着の強化が廃止されました.
結論:
- CAR遺伝子は,粘着信号伝導分子をコードする可能性があります.
- この分子は,細胞-マトリックス相互作用を調節することによって,潜在的に腫瘍の侵入を抑制する機能を持っています.
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